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Genetic Engineering : Principles and Methods - Jane K. Setlow

Genetic Engineering

Principles and Methods

By: Jane K. Setlow (Editor)

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Published: 31st August 2001
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Over the past decade, our laboratory and others have been concerned with molecular archaeological studies aimed at revealing the origins and evolutionary histories of permeases (1). These studies have revealed that several different families, defined on the basis of sequence similarities, arose independently of each other, at different times in evolutionary history, following different routes. When complete microbial genomes first became available for analysis, we adapted p- existing software and designed new programs that allowed us quickly to identify probable transmembrane proteins, estimate their topologies and determine the likelihood that they function in transport (2). This work allowed us to expand previously-recognized families and to identify dozens of new families. All of this work then led us to attempt to design a rational but comprehensive classification system that would be applicable to the complete complement of transport systems found in all living organisms (3). The classification system that we have devised is based primarily on mode of transport and energy coupling mechanism, secondarily on molecular phylogeny, and lastly on the substrate specificities of the individual permeases (4).

Identification and Analysis of Micrornas
Dormancy and the Cell Cycle
The OPT Family Functions in Long Distance Peptide and Metal Transport in Plants
Phospholipid-Derived Signaling in Plant Response to Temperature and Water Stresses
Anionic Nutrient Transport in Plants: The Molecular Basis of Sulfate Transporter Gene Family
Purification of Protein Complexes by Immunoaffinity Chromotography: Application to Transcription Machinery
Biogenesis of Iron-Sulfur Cluster Proteins in Plastids
Iron Transport and Metabolism in Plants
Salt Stress Signaling and Mechanisms of Plant Salt Tolerance
Strategies for High-Throughput Gene Cloning and Expression
Molecular Roles of Chaperones in Assisted Folding and Assembly of Proteins
Engineering Plants for Increased Nutrition and Antioxidant Content through the Manipulation of the Vitamin E Pathway
Index
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780306466458
ISBN-10: 0306466457
Series: Genetic Engineering: Principles and Methods : Book 23
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 232
Published: 31st August 2001
Publisher: SPRINGER VERLAG GMBH
Country of Publication: US
Dimensions (cm): 26.16 x 16.56  x 2.16
Weight (kg): 0.69