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Gene Quantification : Advanced Biomedical Technologies - Francois Ferre

Gene Quantification

Advanced Biomedical Technologies

By: Francois Ferre (Editor)

Hardcover Published: 21st July 1998
ISBN: 9780817639457
Number Of Pages: 375

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Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo- bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num- bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

List of Contributors
Key Issues, Challenges and Future Opportunities in Gene Quantificationp. 1
Present and Future Detection Formats for PCR Quantitation of Nucleic Acidsp. 19
Determination of Target Copy Number of Quantitative Standards Used in PCR Based Diagnostic Assaysp. 31
Quantification of Specific Nucleic Acids, Regulated RNA Processing and Genomic Polymorphisms Using Reversed-Phase HPLCp. 45
Capillary Electrophoresis for Quantitative Genetic Analysisp. 79
Quantitative PCR Technologyp. 97
Statistical Estimations of PCR Amplification Ratesp. 111
Fluorescence Monitoring of Rapid Cycle PCR for Quantificationp. 129
Kinetic Elisa-PCR: A Versatile Quantitative PCR Methodp. 145
Quantitation of RNA by NASBA: Applications and Issues for HIV-1 and AIDSp. 169
Application of Transcription-Mediated Amplification to Quantification of Gene Sequencesp. 189
Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Design and Performancep. 205
Hybrid Capture - A Sensitive Signal Amplified Test for the Detection and Quantitation of Human Viral and Bacterial Pathogensp. 225
Quantification of Gene Expression by Competitive RT-PCR: The hCG[beta]/LH[beta] Gene Clusterp. 253
Quantitative Detection of Mycoplasma DNA Using Competitive PCRp. 265
The Detection and Quantification of bcr-abl in Chronic Myeloid Leukemia Following Marrow Transplantationp. 277
Competitive RT-PCR Analysis of Brain Gene Expression During Inflammation and Diseasep. 295
Development and Application of Real-Time Quantitative PCRp. 313
Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Research and Clinical Applicationsp. 327
Quantification of Plasmid DNA Expression in Vivop. 343
Indexp. 369
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780817639457
ISBN-10: 0817639454
Series: Advanced Biomedical Technologies
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 375
Published: 21st July 1998
Country of Publication: US
Dimensions (cm): 24.28 x 16.26  x 2.36
Weight (kg): 0.71