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Gene Expression Profiling : Methods in Molecular Biology - Richard A. Shimkets

Gene Expression Profiling

Methods in Molecular Biology

By: Richard A. Shimkets (Editor)

Hardcover

Published: 24th February 2004
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Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip (R), SAR-SAGE, StaRT-PCR, SSH, the Invader Assay (R), and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.

Prefacep. v
Contributorsp. xi
Technical Considerations in Quantitating Gene Expressionp. 1
Gene Expression Quantitation Technology Summaryp. 7
Standardized RT-PCR and the Standardized Expression Measurement Centerp. 13
GeneCalling: Transcript Profiling Coupled to a Gene Database Queryp. 43
Invader Assay for RNA Quantitationp. 53
Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarraysp. 71
Amplified Differential Gene Expression Microarrayp. 95
Suppression Subtractive Hybridizationp. 107
Small Amplified RNA-SAGEp. 135
Gene Expression Informaticsp. 153
Indexp. 167
Table of Contents provided by Rittenhouse. All Rights Reserved.

ISBN: 9781588292209
ISBN-10: 1588292207
Series: Methods in Molecular Biology
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 168
Published: 24th February 2004
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 23.5 x 15.5  x 1.91
Weight (kg): 0.47