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Fundamentals of Enzymology : Cell and Molecular Biology of Catalytic Proteins - Nicholas Price

Fundamentals of Enzymology

Cell and Molecular Biology of Catalytic Proteins

Paperback Published: 11th November 1999
ISBN: 9780198502296
Number Of Pages: 496

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Since the publication of the successful and popular second edition of Fundamentals of Enzymology in 1989, a large increase in the knowledge of several aspects of enzymology has occurred -- particularly in the areas of structural characterization of enzymes and bioinformatics. This new edition places appropriate emphasis on the new knowledge while consolidating the strengths of the previous editions. As before, this volume gives a thorough overview of the field that includes: enzyme purification and characterization; enzyme structure (including information on World Wide Web sites); enzyme kinetics; the mechanisms and control of enzyme action; enzyme folding; how enzymes act in vivo; enzyme synthesis and degradation; and clinical and industrial applications of enzymology. Furthermore, the integration of these themes is stressed throughout the book, making it useful for advanced undergraduates in biochemistry and molecular biology.

`...Fundamentals of Enzymology remains an extremely useful textbook for undergraduate students and their teachers of enzymology and is a useful reference work for postgraduate students embarking on research in this area.' Trends in Biochemical Sciences

A note on unitsp. xv
List of abbreviationsp. xvii
Introductionp. 1
Aims of this bookp. 1
Historical aspectsp. 1
Remarkable properties of enzymes as catalystsp. 4
Cofactorsp. 7
Nomenclature and classification of enzymesp. 8
The contents of this bookp. 13
Referencesp. 14
The purification of enzymesp. 15
Introductionp. 15
Why isolate enzymes?p. 15
Objectives and strategy in enzyme purificationp. 16
Choice of sourcep. 17
Methods of homogenizationp. 20
Methods of separationp. 21
How to judge the success of a purification procedurep. 34
Examples of purification proceduresp. 36
Conclusions from the examples of enzyme purificationp. 43
Referencesp. 44
The structure of enzymesp. 47
Introductionp. 47
The determination of M[subscript r]p. 48
The determination of amino-acid composition and primary structurep. 54
The determination of secondary and tertiary structurep. 75
The determination of quaternary structurep. 100
The unfolding and folding of enzymesp. 106
Concluding remarksp. 110
Referencesp. 111
Appendix 3.1p. 116
References for Appendix 3.1p. 117
An introduction to enzyme kineticsp. 118
Outline of the chapterp. 118
How do we obtain kinetic data?p. 119
How do we analyse kinetic data?p. 121
Pre-steady-state kineticsp. 144
Concluding remarksp. 148
Referencesp. 148
Appendix 4.1p. 150
Appendix 4.2p. 151
Appendix 4.3p. 152
References for Appendicesp. 153
The mechanism of enzyme actionp. 154
Introductionp. 154
Definition of the mechanism of an enzyme-catalysed reactionp. 154
Background to catalysisp. 155
Experimental approaches to the determination of enzyme mechanismsp. 162
Examples of enzyme mechanismsp. 177
Concluding comments on enzyme mechanismsp. 212
Referencesp. 212
The control of enzyme activityp. 217
Introductionp. 217
Control of the activities of single enzymesp. 218
Control of metabolic pathwaysp. 237
Examples of control of metabolic pathwaysp. 249
Concluding remarksp. 266
Referencesp. 266
Appendix 6.1p. 270
Enzymes in organized systemsp. 273
Introductionp. 273
Organized enzyme systemsp. 273
RNA polymerase from E. colip. 274
The occurrence and isolation of multienzyme proteinsp. 281
Phylogenetic distribution of multienzyme proteinsp. 282
Properties of multienzyme proteinsp. 283
Pyruvate dehydrogenase multienzyme complex and related systemsp. 285
Glycine decarboxylase multienzyme complexp. 294
The tryptophan synthase multienzyme complex from E. colip. 294
Carbamoyl phosphate synthase and the associated enzymes of the pyrimidine and arginine biosynthetic pathways in E. coli, fungi, and mammalian cellsp. 300
Multienzyme polypeptides: fatty-acid synthase and the arom complex (AROM enzyme)p. 304
Enzymes involved in DNA synthesisp. 315
The glycogen particlep. 315
Conclusionsp. 317
Referencesp. 317
Enzymes in the cellp. 321
Introductionp. 321
Intracellular compartmentationp. 322
Compartmentation of metabolic pathwaysp. 333
Vectorial organization of enzymes associated with membranesp. 347
The concentrations of enzymes and substrates in vivop. 355
Conclusionsp. 366
Referencesp. 367
Enzyme turnoverp. 370
Introductionp. 370
Kinetics of enzyme turnoverp. 371
Methods for measurement of rates of enzyme turnoverp. 373
Results from measurements of rates of enzyme turnoverp. 375
Possible correlations between the rates of turnover and the structure and function of enzymesp. 376
The mechanisms of protein degradationp. 377
The significance of enzyme turnoverp. 389
Other processes in which intracellular proteolysis is importantp. 394
Conclusionsp. 396
Referencesp. 396
Appendix 9.1p. 399
Clinical aspects of enzymologyp. 400
Introductionp. 400
Determination of enzyme activities for clinical diagnosisp. 401
Clinical enzymology of liver diseasep. 404
Clinical enzymology of heart diseasep. 406
Other enzyme activities that become elevated in serum in diseasep. 408
The detection and significance of enzyme deficienciesp. 411
Enzyme inhibitors and drug designp. 415
The use of enzymes to determine the concentrations of metabolites of clinical importancep. 416
Enzyme therapyp. 424
Conclusionsp. 428
Referencesp. 428
Enzyme technologyp. 430
Introductionp. 430
Use of microorganisms in brewing and cheesemakingp. 430
Use of microorganisms in the production of organic chemicalsp. 433
Use of isolated enzymes in industrial processesp. 433
Immobilized enzymesp. 440
Referencesp. 449
Enzymes referred to in Chapters 1-11p. 450
Indexp. 471
Table of Contents provided by Syndetics. All Rights Reserved.

ISBN: 9780198502296
ISBN-10: 019850229X
Audience: Tertiary; University or College
Format: Paperback
Language: English
Number Of Pages: 496
Published: 11th November 1999
Publisher: Oxford University Press
Country of Publication: GB
Dimensions (cm): 24.6 x 18.9  x 2.9
Weight (kg): 1.15
Edition Number: 3
Edition Type: Revised