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Fluorescence Spectroscopy : Volume 278 - John N. Abelson

Fluorescence Spectroscopy

Volume 278

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Ludwig Brand (Editor), Michael L. Johnson (Editor)

Hardcover Published: 1st May 1997
ISBN: 9780121821791
Number Of Pages: 628

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Fluorescence in biophysics
* Fluorescence as a tool for studying proteins
* Determination of ground-state dissociation constant by fluorescence spectroscopy
* Fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions
* Fluorescence methods for studying equilibrium macromolecule-liquid interactions
* Methods for detecting molecular clusters on cell surfaces

Industry Reviews

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

"Incomparably useful." --ANALYTICAL BIOCHEMISTRY

"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY

"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Fluorescence in Biophysics: Accomplishments and Deficiencies
Design of Profluorescent Protease Substrates Guided by Exciton Theory
Picosecond Fluorescence Decay Curves Collected on Millisecond Time Scale: Direct Measurement of Hydrodynamic Radii, Local/Global Mobility, and Intramolecular Distances during Protein Folding Reactions
Time-Resolved Room Temperature Tryptophan Phosphorescence in Proteins
Fluorescence Line Narrowing Spectroscopy: A Tool for Studying Proteins
Determination of Ground-State Dissociation Constant by Fluorescence Spectroscopy
1La and 1Lb Transitions of Tryptophan: Applications of Theory and Experimental Observations to Fluorescence of Proteins
Enhancement of Protein Spectra with Tryptophan Analogues: Fluorescence Spectroscopy of ProteinProtein and ProteinNucleic Acid Interactions
Time-Resolved Fluorescence of Constrained Tryptophan Derivatives: Implications for Protein Fluorescence
Conformational Heterogeneity in Crystalline Proteins: Time-Resolved Fluorescence Studies
Fluorescence Methods for Studying Equilibrium Macromolecule-Ligand Interactions
Fluorescence Methods for Studying Kinetics of Protein Folding Reactions
Intramolecular Pyrene Excimer Fluorescence: A Probe of Proximity and Protein Conformational Change
Long Lifetime Metal-Ligand Complexes as Probes in Biophysics and Clinical Chemistry
N-Terminal Modification of Proteins for Fluorescence Measurements
Fluorescence Studies of Zinc Finger Peptides and Proteins
Fluorescence Assays for DNA Cleavage
Fluorescent Nucleotide Analogs: Synthesis and Applications
Fluorescence Approaches to Study of Protein-Nucleic Acid Complexation
Fluorescence Resonance Energy Transfer as Probe of DNA Structure and Function
Energy Transfer Methods for Detecting Molecular Clusters on Cell Surfaces
Distribution Analysis of Depth-Dependent Fluorescence Quenching in Membranes: A Practical Guide
Mechanism of Leakage of Contents of Membrane Vesicles Determined by Fluorescence Requenching
Fluorescence Probes for Studying Membrane Heterogeneity
Preparation of Bifluorescent-Labeled Glycopeptides for Glycoamidase Assay
Preparation of Fluorescent-Labeled Neoglycolipids for Ceramide Glycanase Assays
Applications of Fluorescence Energy Resonance Transfer to Structure and Mechanism of Chloroplast ATPSynthase
Intrinsic Fluorescence of Hemoglobins and Myoglobins
Multiple-Domain Fluorescence Lifetime Data Analysis
Author Index
Subject Index
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121821791
ISBN-10: 012182179X
Series: Methods in Enzymology : Book 278
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 628
Published: 1st May 1997
Country of Publication: US
Dimensions (cm): 22.91 x 15.19  x 3.51
Weight (kg): 1.04