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Fluorescence in Situ Hybridization (FISH) : Protocols and Applications - Joanna M. Bridger

Fluorescence in Situ Hybridization (FISH)

Protocols and Applications

By: Joanna M. Bridger (Editor), Keith Morris (Editor)

Hardcover Published: 9th September 2010
ISBN: 9781607617884
Number Of Pages: 451

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The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.

Industry Reviews

From the reviews:

"Fluorescence in situ hybridization (FISH) has been widely adopted to enable the study of uncultured target cells. This book shows many more applications in cytology. Relatively few chapters are devoted directly to microbiological applications; the strong theme of the book describes methods targeting chromosomes. ... Protocols on 3-D FISH may have useful material for microbiologists, e.g. studies on biofilms. ... I would suggest this is for microbiological libraries ... ." (Jonathan Porter, Microbiology Today, November, 2011)

Prefacep. v
Contributorsp. xi
Core Techniques
Fluorescence in situ Hybridization (FISH), Basic Principles and Methodologyp. 3
Fluorescence In Situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibresp. 21
Detection of Nascent RNA Transcripts by Fluorescence in Situ Hybridizationp. 33
Fluorescence In Situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarraysp. 51
Fluorescence in situ Hybridization (FISH) on Tissue Cryosectionsp. 71
Multiplex Fluorescence in situ Hybridization (M-FISH)p. 83
Optical Mapping of Protein-DNA Complexes on Chromatin Fibersp. 99
3D-FISH on Cultured Cells Combined with Immunostainingp. 117
Technical Advancements and Novel Adaptations
The Comet-FISH Assay for the Analysis of DNA Damage and Repairp. 129
Direct In Situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probesp. 147
LNA-FISH for Detection of MicroRNAs in Frozen Sectionsp. 165
Chromosome Orientation Fluorescence In Situ Hybridization or Strand-Specific FISHp. 173
Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cellsp. 185
Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturationp. 203
CryoFISH: Fluorescence In Situ Hybridization on Ultrathin Cryosectionsp. 219
Characterization of Chromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)p. 231
Visualizing Nucleic Acids in Living Cells by Fluorescence In Vivo Hybridizationp. 239
Translational FISH: Applications for Human Genetics and Medicine
Quality Control in FISH as Part of a Laboratory's Quality Management Systemp. 249
Flash FISH: "Same Day" Prenatal Diagnosis of Common Chromosomal Aneuploidiesp. 261
FISH for Pre-implantation Genetic Diagnosisp. 269
PNA-FISH on Human Spermp. 283
POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphismp. 291
Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)p. 299
ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Materialp. 313
Fluorescence In Situ Hybridization on 3D Cultures of Tumor Cellsp. 323
Simultaneous Ultrasensitive Subpopulation Staining/Hybridization In Situ (SUSHI) in HIV-1 Disease Monitoringp. 337
Protocols for Model Organisms
Detection of Prokaryotic Cells with Fluorescence In Situ Hybridizationp. 349
FISH as a Tool to Investigate Chromosome Behavior in Budding Yeastp. 363
FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabratap. 379
Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic Heterochromatin of Drosophilap. 389
Three-Dimensional Fluorescence In Situ Hybridization in Mouse Embryos Using Repetitive Probe Sequencesp. 401
Fluorescence in situ Hybridization (FISH) for Genomic Investigations in Ratp. 409
Fluorescence In Situ Hybridization on Early Porcine Embryosp. 427
FISH on 3D Preserved Bovine and Murine Preimplantation Embryosp. 437
Indexp. 447
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9781607617884
ISBN-10: 1607617889
Series: Methods in Molecular Biology : Book 659
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 451
Published: 9th September 2010
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 26.04 x 18.42  x 3.18
Weight (kg): 1.02