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Eukaryotic DNA Replication: A Practical Approach is a comprehensive practical manual, with each of its eleven chapters describing an aspect of the methods currently used to investigate DNA replication in eukaryotes. The sequence of the chapters corresponds roughly to the order of events during DNA replication. The first chapters are concerned with initiation, looking at methods to characterize origins of replication and the proteins that interact with them. There then follow chapters describing protocols for the study of the elongation phase and the synthesis of the telomeres. The final chapters provide a more general overview of the study of DNA replication - including its investigation in model systems such as yeast, xenopus and viruses, and looks into methods used to study DNA:protein interactions that could be applied to the study of replication proteins. This exciting new volume provides over 120 tried and tested protocols for the analysis of eukaryotic DNA replication and will be of major interest to a wide variety of molecular and cell biologists, biochemists and medical researchers.
|List of contributors|
|Methods to map origins of replication in eukaryotes|
|Purification of yeast replication origin binding proteins|
|Eukaryotic DNA polymerases|
|Identification and characterization of DNA helicases|
|Functional analysis of DNA replication accessory proteins|
|Isolation and characterization of lagging strand processing activities|
|Analysis of telomere replication activities: telomerase and telomere binding proteins|
|Saccharomyces cerevisiae as a model system to study DNA replication|
|The use of Xenopus laevis interphase egg extracts to study genomic DNA replication|
|Viral in vitro replication systems|
|Analysis of DNA replication complexes by DNA probing|
|Table of Contents provided by Publisher. All Rights Reserved.|
Series: Practical Approach Series
Number Of Pages: 304
Published: 1st June 1999
Country of Publication: GB
Dimensions (cm): 23.5 x 15.6 x 1.6
Weight (kg): 0.52