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Eukaryotic DNA Replication : A Practical Approach - Sue Cotterill

Eukaryotic DNA Replication

A Practical Approach

By: Sue Cotterill (Editor)

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Eukaryotic DNA Replication: A Practical Approach is a comprehensive practical manual, with each of its eleven chapters describing an aspect of the methods currently used to investigate DNA replication in eukaryotes. The sequence of the chapters corresponds roughly to the order of events during DNA replication. The first chapters are concerned with initiation, looking at methods to characterize origins of replication and the proteins that interact with them. There then follow chapters describing protocols for the study of the elongation phase and the synthesis of the telomeres. The final chapters provide a more general overview of the study of DNA replication - including its investigation in model systems such as yeast, xenopus and viruses, and looks into methods used to study DNA:protein interactions that could be applied to the study of replication proteins. This exciting new volume provides over 120 tried and tested protocols for the analysis of eukaryotic DNA replication and will be of major interest to a wide variety of molecular and cell biologists, biochemists and medical researchers.

List of contributors
Methods to map origins of replication in eukaryotes
Purification of yeast replication origin binding proteins
Eukaryotic DNA polymerases
Identification and characterization of DNA helicases
Functional analysis of DNA replication accessory proteins
Isolation and characterization of lagging strand processing activities
Analysis of telomere replication activities: telomerase and telomere binding proteins
Saccharomyces cerevisiae as a model system to study DNA replication
The use of Xenopus laevis interphase egg extracts to study genomic DNA replication
Viral in vitro replication systems
Analysis of DNA replication complexes by DNA probing
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780199636808
ISBN-10: 019963680X
Series: Practical Approach Series
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 304
Published: 1st June 1999
Country of Publication: GB
Dimensions (cm): 22.86 x 15.24  x 1.27
Weight (kg): 0.52