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Erythrocyte Engineering for Drug Delivery and Targeting : Biotechnology Intelligence Unit - Mauro Magnani

Erythrocyte Engineering for Drug Delivery and Targeting

Biotechnology Intelligence Unit

By: Mauro Magnani (Editor)

Hardcover

Published: 31st January 2003
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The International Symposia on Plant Lipids, the 15th of which was held in Okazaki, Japan, in May 12-17, 2002, is held every two years and is the only international meeting in this field. The contributions from the symposium collected in this book represent the most up-to-date research results on plant lipids,including their structure, analysis, biosynthesis, regulation, physiological function, environmental aspects, and biotechnology, obtained world-wide during 2000-2002.

Prefacep. viii
Red Blood Cell Loading: A Selection of Proceduresp. 1
Introductionp. 1
Main Procedures for the Entrapment of Drugs in Erythrocytesp. 1
Electroporationp. 2
Development of Apparatus for Clinical Applications of Loaded Erythrocytesp. 10
Conclusionsp. 15
Towards Activated Release of Payloads from Carrier Erythrocytesp. 19
Introductionp. 19
Current Exploitation of Erythrocytes in Delivery and Targetingp. 19
The Case for an Activatable Payload Release Mechanism from Carrier Erythrocytesp. 20
Photodynamic Activation and Activated Payload Release from a Light-Sensitive Erythrocyte Carrier Systemp. 21
Electric Field-Mediated Stimulation of Payload Release from HPD-Sensitized Human Erythrocytesp. 23
Challenges Associated with Activated Release from Erythrocyte Carriersp. 25
Targeting Drug Loaded Red Blood Cellsp. 29
Introductionp. 29
Red Blood Cells as Drug Delivery Systemp. 30
Targeting Drug-Loaded Red Blood Cellsp. 30
Drug Targeting to Macrophages by Carrier Red Blood Cellsp. 31
Targeting New Anti-Viral Drugsp. 31
Targeting Corticosteroid Analoguesp. 32
Targeting Peptidesp. 33
Conclusionsp. 34
Streptavidin-Mediated Coupling of Therapeutic Proteins to Carrier Erythrocytesp. 37
Introduction: RBC as Drug Carrierp. 37
Loading of Drugs Inside RBC and Coupling of Therapeutic Proteins to RBC Surfacep. 38
Destruction and Elimination of Modified RBC by Complement and Phagocytesp. 39
Biocompatibility of RBC Modified with Non-Specific Cross-Linkersp. 41
Coupling of Active Therapeutic Proteins to RBC via Streptavidin-Biotinp. 43
Biocompatibility of RBC Modified with Biotin and (strept)Avidin: In vitro Studiesp. 47
Biocompatibility of RBC Modified with Biotin and Streptavidin: In vivo Studiesp. 49
Prolonged Circulation of Therapeutic Proteins Coupled to RBCp. 55
Conclusion and Perspectivesp. 58
Vaccination Strategy Using Red Blood Cells as Antigen Delivery Systemp. 69
Introductionp. 69
Immobilization of Antigen on Red Blood Cellsp. 70
Immunological Response to RBC Coupled with Proteinsp. 71
Immunological Response to RBC Coupled with Recombinant Proteinsp. 71
Immunological Response to RBC Coupled with Surface Particles of Virusp. 72
Antigen Delivery System for Human Dendritic Cellsp. 72
Conclusionsp. 73
Engineered Nanoerythrocytes as a Novel Drug Delivery Systemp. 77
Introductionp. 77
Advantagesp. 78
The Erythrocyte and Erythrocyte Membranep. 78
Requirements for Encapsulationp. 78
Isolation of Erythrocytes, Preparation of Erythrocyte Ghosts and Nanoerythrocytesp. 79
Inside-Out Red Cell Membraneous Vesiclesp. 81
In vitro Characterizationp. 83
Routes of Administrationp. 85
Stability Studiesp. 86
In vivo Studies and Toxicityp. 87
Immunological Considerationsp. 89
Targeting Potential and Applications of Nanoerythrocytesp. 90
Advances, Conclusion and Future Prospectsp. 90
Red Blood Cells as Carriers of Antiviral Agentsp. 93
Introductionp. 93
Red Blood Cells for a Slow Release of Antiviral Drugsp. 93
Red Blood Cells for Targeting of Antiviral Drugsp. 94
Red Blood Cells as Carriers of Antiretroviral and Antiherpetic Drugsp. 96
Conclusionsp. 97
Erythrocytes as Carriers of Anthracycline Antibiotics in vitro and in vivop. 103
Introductionp. 103
Anthracycline Antibioticsp. 103
Carrier Macromoleculesp. 104
Liposomesp. 104
Carrier Erythrocytesp. 105
Conclusionsp. 109
Drug-Loaded Red Blood Cells for the Control of the Inflammatory Response: Selective Targeting of Nuclear Factor-[kappa]B (NF-[kappa]B)p. 113
Introductionp. 113
Targeting Glucocorticoids to Macrophages: Inhibition of NF-[kappa]B Activation and Cytokine Releasep. 114
Targeting Ubiquitin Analogues to Macrophages: A New Approach to Interfering with NF-[kappa]B Activationp. 115
Conclusionsp. 119
Design and Synthesis of New Pro-Drugs to be Used with Carrier Red Blood Cellsp. 123
Introductionp. 123
Dimeric Fluoropyrimidine Synthesis and Intraerythrocytic Biochemical Pathwayp. 124
AZTp2AZT Homodinucleotide Synthesis and Characterizationp. 126
AZTp2ACV Heterodinucleotide Synthesis and Characterizationp. 128
AZTp2EMB Heterodinucleotide Synthesis and Characterizationp. 130
Conclusionsp. 131
Engineered Red Blood Cells as Circulating Bioreactorsp. 135
Introductionp. 135
The Entrapped Enzymesp. 136
Coentrapment of Two Enzymesp. 139
Enzymes Bound to RBC Membranep. 139
Enzymes Encapsulated in RBC to Lower Alcohol Toxicityp. 141
Conclusionp. 142
Indexp. 147
Table of Contents provided by Syndetics. All Rights Reserved.

ISBN: 9780306476914
ISBN-10: 0306476916
Series: Biotechnology Intelligence Unit
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 148
Published: 31st January 2003
Publisher: Springer Science+Business Media
Country of Publication: US
Dimensions (cm): 23.5 x 15.5  x 1.91
Weight (kg): 0.91
Edition Type: Illustrated