

Hardcover
Published: 31st January 2003
ISBN: 9780306476914
Number Of Pages: 148
Earn 678 Qantas Points
on this Book
The International Symposia on Plant Lipids, the 15th of which was held in Okazaki, Japan, in May 12-17, 2002, is held every two years and is the only international meeting in this field. The contributions from the symposium collected in this book represent the most up-to-date research results on plant lipids,including their structure, analysis, biosynthesis, regulation, physiological function, environmental aspects, and biotechnology, obtained world-wide during 2000-2002.
Preface | p. viii |
Red Blood Cell Loading: A Selection of Procedures | p. 1 |
Introduction | p. 1 |
Main Procedures for the Entrapment of Drugs in Erythrocytes | p. 1 |
Electroporation | p. 2 |
Development of Apparatus for Clinical Applications of Loaded Erythrocytes | p. 10 |
Conclusions | p. 15 |
Towards Activated Release of Payloads from Carrier Erythrocytes | p. 19 |
Introduction | p. 19 |
Current Exploitation of Erythrocytes in Delivery and Targeting | p. 19 |
The Case for an Activatable Payload Release Mechanism from Carrier Erythrocytes | p. 20 |
Photodynamic Activation and Activated Payload Release from a Light-Sensitive Erythrocyte Carrier System | p. 21 |
Electric Field-Mediated Stimulation of Payload Release from HPD-Sensitized Human Erythrocytes | p. 23 |
Challenges Associated with Activated Release from Erythrocyte Carriers | p. 25 |
Targeting Drug Loaded Red Blood Cells | p. 29 |
Introduction | p. 29 |
Red Blood Cells as Drug Delivery System | p. 30 |
Targeting Drug-Loaded Red Blood Cells | p. 30 |
Drug Targeting to Macrophages by Carrier Red Blood Cells | p. 31 |
Targeting New Anti-Viral Drugs | p. 31 |
Targeting Corticosteroid Analogues | p. 32 |
Targeting Peptides | p. 33 |
Conclusions | p. 34 |
Streptavidin-Mediated Coupling of Therapeutic Proteins to Carrier Erythrocytes | p. 37 |
Introduction: RBC as Drug Carrier | p. 37 |
Loading of Drugs Inside RBC and Coupling of Therapeutic Proteins to RBC Surface | p. 38 |
Destruction and Elimination of Modified RBC by Complement and Phagocytes | p. 39 |
Biocompatibility of RBC Modified with Non-Specific Cross-Linkers | p. 41 |
Coupling of Active Therapeutic Proteins to RBC via Streptavidin-Biotin | p. 43 |
Biocompatibility of RBC Modified with Biotin and (strept)Avidin: In vitro Studies | p. 47 |
Biocompatibility of RBC Modified with Biotin and Streptavidin: In vivo Studies | p. 49 |
Prolonged Circulation of Therapeutic Proteins Coupled to RBC | p. 55 |
Conclusion and Perspectives | p. 58 |
Vaccination Strategy Using Red Blood Cells as Antigen Delivery System | p. 69 |
Introduction | p. 69 |
Immobilization of Antigen on Red Blood Cells | p. 70 |
Immunological Response to RBC Coupled with Proteins | p. 71 |
Immunological Response to RBC Coupled with Recombinant Proteins | p. 71 |
Immunological Response to RBC Coupled with Surface Particles of Virus | p. 72 |
Antigen Delivery System for Human Dendritic Cells | p. 72 |
Conclusions | p. 73 |
Engineered Nanoerythrocytes as a Novel Drug Delivery System | p. 77 |
Introduction | p. 77 |
Advantages | p. 78 |
The Erythrocyte and Erythrocyte Membrane | p. 78 |
Requirements for Encapsulation | p. 78 |
Isolation of Erythrocytes, Preparation of Erythrocyte Ghosts and Nanoerythrocytes | p. 79 |
Inside-Out Red Cell Membraneous Vesicles | p. 81 |
In vitro Characterization | p. 83 |
Routes of Administration | p. 85 |
Stability Studies | p. 86 |
In vivo Studies and Toxicity | p. 87 |
Immunological Considerations | p. 89 |
Targeting Potential and Applications of Nanoerythrocytes | p. 90 |
Advances, Conclusion and Future Prospects | p. 90 |
Red Blood Cells as Carriers of Antiviral Agents | p. 93 |
Introduction | p. 93 |
Red Blood Cells for a Slow Release of Antiviral Drugs | p. 93 |
Red Blood Cells for Targeting of Antiviral Drugs | p. 94 |
Red Blood Cells as Carriers of Antiretroviral and Antiherpetic Drugs | p. 96 |
Conclusions | p. 97 |
Erythrocytes as Carriers of Anthracycline Antibiotics in vitro and in vivo | p. 103 |
Introduction | p. 103 |
Anthracycline Antibiotics | p. 103 |
Carrier Macromolecules | p. 104 |
Liposomes | p. 104 |
Carrier Erythrocytes | p. 105 |
Conclusions | p. 109 |
Drug-Loaded Red Blood Cells for the Control of the Inflammatory Response: Selective Targeting of Nuclear Factor-[kappa]B (NF-[kappa]B) | p. 113 |
Introduction | p. 113 |
Targeting Glucocorticoids to Macrophages: Inhibition of NF-[kappa]B Activation and Cytokine Release | p. 114 |
Targeting Ubiquitin Analogues to Macrophages: A New Approach to Interfering with NF-[kappa]B Activation | p. 115 |
Conclusions | p. 119 |
Design and Synthesis of New Pro-Drugs to be Used with Carrier Red Blood Cells | p. 123 |
Introduction | p. 123 |
Dimeric Fluoropyrimidine Synthesis and Intraerythrocytic Biochemical Pathway | p. 124 |
AZTp2AZT Homodinucleotide Synthesis and Characterization | p. 126 |
AZTp2ACV Heterodinucleotide Synthesis and Characterization | p. 128 |
AZTp2EMB Heterodinucleotide Synthesis and Characterization | p. 130 |
Conclusions | p. 131 |
Engineered Red Blood Cells as Circulating Bioreactors | p. 135 |
Introduction | p. 135 |
The Entrapped Enzymes | p. 136 |
Coentrapment of Two Enzymes | p. 139 |
Enzymes Bound to RBC Membrane | p. 139 |
Enzymes Encapsulated in RBC to Lower Alcohol Toxicity | p. 141 |
Conclusion | p. 142 |
Index | p. 147 |
Table of Contents provided by Syndetics. All Rights Reserved. |
ISBN: 9780306476914
ISBN-10: 0306476916
Series: Biotechnology Intelligence Unit
Audience:
General
Format:
Hardcover
Language:
English
Number Of Pages: 148
Published: 31st January 2003
Publisher: Springer Science+Business Media
Country of Publication: US
Dimensions (cm): 23.65 x 16.1
x 1.98
Weight (kg): 0.38
Edition Type: Illustrated
Earn 678 Qantas Points
on this Book