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Epithelial Transport : A Guide to Methods and Experimental Analysis - Nancy K. Wills

Epithelial Transport

A Guide to Methods and Experimental Analysis

Hardcover Published: 31st July 1996
ISBN: 9780412434006
Number Of Pages: 356

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How is the epithelia organized? Which molecules mediate ion transport? How is an epithelia maintained? In this volume the authors discuss these and other key questions in the field and provide a clear guide to the methods used to answer them. The complexity of epithelial transport is reflected in the diverse approaches and methodologies used in its study. Written for active researchers, this book draws on the laboratory experience of leading investigators. An overview of the general principles underlying epithelial transport of ions, water and non-electrolytes is combined with guidance on practical approaches. The relative strengths and weaknesses of current methodologies are emphasized and potential pitfalls highlighted. This book should be of interest to graduates and researchers in cell biology, physiology, pharmacology, neurosciences and molecular biology.

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...it will certainly attract not only the undergraduate and graduate students, but also post-doctoral fellows and established investigators. We think it will be particularly useful for clinicians and dermatologists... - Cellular and Molecular Biology

List of contributors
Epithelial structure and functionp. 1
Epithelial transport proteinsp. 21
Epithelial polarityp. 49
Characterization of epithelial ion transportp. 70
Epithelial electrophysiologyp. 93
Impedance analysis of epitheliap. 118
Measurements and interpretation of cytoplasmic ion activitiesp. 146
Measurements of epithelial cell volumep. 167
Methods and experimental analysis of isolated epithelial cell membranesp. 190
Methods and experimental analysis of single ion channelsp. 212
Epithelial cell culturep. 236
Signaling pathways regulating ion transport in polarized cellsp. 256
The cytoskeleton and epithelial functionp. 275
Future perspectives: molecular biology and pathophysiologyp. 306
Appendix A: Instrumentationp. 323
Appendix B: Solving simple equivalent circuitsp. 325
Appendix C: A spread-sheet for marker distribution datap. 329
Indexp. 335
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780412434006
ISBN-10: 0412434008
Audience: General
Format: Hardcover
Language: English
Number Of Pages: 356
Published: 31st July 1996
Publisher: Chapman and Hall
Country of Publication: GB
Dimensions (cm): 24.08 x 15.65  x 2.87
Weight (kg): 0.81

Earn 746 Qantas Points
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