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Enzyme Kinetics and Mechanism, Part D : Developments in Enzyme Dynamics: Volume 249 - John N. Abelson

Enzyme Kinetics and Mechanism, Part D

Developments in Enzyme Dynamics: Volume 249

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Daniel Purich (Editor)

Hardcover Published: 5th April 1995
ISBN: 9780121821500
Number Of Pages: 662

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General Description of the Volume:
This volume, as do the other "Enzyme Kinetics and Mechanism" volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Mechanisms of enzyme catalysis and inhibition
* Enzyme structure and function
* Regulatory control of enzymes
* Action of catalytic antibodies and ribozymes

Industry Reviews

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

"Incomparably useful." --ANALYTICAL BIOCHEMISTRY

"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY

"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

General Approaches to Biological Catalysis
Transient Kinetic Approaches to Enzyme Mechanisms
Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates
Analysis of Enzyme Progress Curves by Nonlinear Regression
Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis
Inhibitors as Mechanistic Probes
Reversible Enzyme Inhibitors as Mechanistic Probes
Kinetics of Slow and Tight-Binding Inhibitors
Kinetic Method for Determination of Dissociation Constants of Metal Ion(Nucleotide Complexes
Product Inhibition Applications
Kinetics of Iso Mechanisms
Mechanism-Based Enzyme Inactivators
Transition State and Multisubstrate Analog Inhibitors
Isotopic Probles of Enzyme Action
Partition Analysis: Detecting Enzyme Reaction Cycle Intermediates
Isotope Effects: Determination of Enzyme Transition State Structure
Hydrogen Tunneling in Enzyme Catalysis
Positional Isotope Exchange as Probe of Enzyme Action
Manipulating Phosphorus Stereospecificity of Adenylate Kinase by Site-Directed Mutagenesis
Equilibrium Isotope Exchange in Enzyme Catalysis
Proton Transfer in Carbonic Anhydrase Measured by Equilibrium Isotope Exchange
Kinetics of Specialized Systems
Expression of Properly Folded Catalytic Antibodies in Escherichia coli
Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects
Kinetic Basis for Interfacial Catalysis by Phospholipase A2
Author Index
Subject Index
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121821500
ISBN-10: 0121821501
Series: Methods in Enzymology : Book 249
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 662
Published: 5th April 1995
Country of Publication: US
Dimensions (cm): 23.62 x 15.95  x 3.3
Weight (kg): 1.02

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