This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.
An Introduction to ELISA (D. Kemeny & S. Chanter).
Microtitre Plates and Other Solid Phase Supports (D. Kemeny & S. Challacombe).
Quantitative Aspects of Solid Phase Immunoassays (R. Hamilton & N. Atkinson).
Amplification by Second Enzymes (A. Johannsson & D. Bates).
The Amplified ELISA (a-ELISA): Immunochemistry and Applications (J. Butler).
The Role of Antibody Affinity in the Performance of Solid Phase Assays (M. Devey & M. Steward).
The Immunochemistry of Sandwich ELISAs: Principles and Applications for the Quantitative Determination of Immunoglobulins (J. Butler).
The Use of ELISA in the Characterization of Protein Antigen Structure and Immune Response (A. Pesce & J. Michael).
The Modified Sandwich ELISA (SELISA) for the Detection of IgE and Other Antibody Isotypes (D. Kemeny).
The Solid Phase Enzyme-Linked Immunospot Assay (ELISPOT) for Enumerating Antibody-Secreting Cells: Methodology and Applications (C. Czerkinsky, et al.).
ELISA-Plaque Assay for the Detection of Single Antibody-Secreting Cells (J. Sedgwick & P. Holt).
Chemiluminescence Immunoassay (I. Weeks & J. Woodhead).
APPLICATIONS TO MICROBIOLOGY.
The Use of ELISA for Rapid Viral Diagnosis: Viral Antigen Detection in Clinical Specimens (S. Chantler & A. Clayton).
The Use of ELISA for Rapid Viral Diagnosis: Antibody Detection (R. Duncan).
Application of ELISA to Bacteriology (S. Challacombe).
Series: Wiley Medical Publications
Number Of Pages: 378
Published: 8th January 1991
Country of Publication: GB
Dimensions (cm): 22.95 x 15.35
Weight (kg): 0.57
Edition Number: 1