ELISA and Other Solid Phase Immunoassays

BASIC ASPECTS.

An Introduction to ELISA (D. Kemeny & S. Chanter).

Microtitre Plates and Other Solid Phase Supports (D. Kemeny & S. Challacombe).

Quantitative Aspects of Solid Phase Immunoassays (R. Hamilton & N. Atkinson).

AMPLIFICATION.

Amplification by Second Enzymes (A. Johannsson & D. Bates).

The Amplified ELISA (a-ELISA): Immunochemistry and Applications (J. Butler).

MOLECULAR ASPECTS.

The Role of Antibody Affinity in the Performance of Solid Phase Assays (M. Devey & M. Steward).

The Immunochemistry of Sandwich ELISAs: Principles and Applications for the Quantitative Determination of Immunoglobulins (J. Butler).

The Use of ELISA in the Characterization of Protein Antigen Structure and Immune Response (A. Pesce & J. Michael).

The Modified Sandwich ELISA (SELISA) for the Detection of IgE and Other Antibody Isotypes (D. Kemeny).

CELLULAR ELISAs.

The Solid Phase Enzyme-Linked Immunospot Assay (ELISPOT) for Enumerating Antibody-Secreting Cells: Methodology and Applications (C. Czerkinsky, et al.).

ELISA-Plaque Assay for the Detection of Single Antibody-Secreting Cells (J. Sedgwick & P. Holt).

CHEMILUMINESCENCE.

Chemiluminescence Immunoassay (I. Weeks & J. Woodhead).

APPLICATIONS TO MICROBIOLOGY.

The Use of ELISA for Rapid Viral Diagnosis: Viral Antigen Detection in Clinical Specimens (S. Chantler & A. Clayton).

The Use of ELISA for Rapid Viral Diagnosis: Antibody Detection (R. Duncan).

Application of ELISA to Bacteriology (S. Challacombe).

Appendix.

Index.