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DNA Microarrays : A Practical Approach - Mark Schena

DNA Microarrays

A Practical Approach

By: Mark Schena (Editor)

Paperback

Published: 1st August 1999
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DNA Microarrays: A Practical Approach is the first comprehensive overview of an exciting and powerful new technology. DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA, providing a massive parallel platform for data gathering and representing a fundamental technical advance in biomedical research. Such biochips gather data at an unprecedented rate by enabling the use of advanced fabrication, detection, and data mining technologies. Written and edited by experts in the field, this book provides fascinating insight into this remarkable advancement. It opens with an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips, and continues with descriptions of the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. The chapters address many topics, among them assay optimization, antisense scanning arrays, the manufacture of molecular arrays, and gene expression analysis. Also addressed are the uses of expression data in bioinformatics, of active microelectronic arrays for DNA hybridization analysis, and of microarray technology in pharmacogenomics. DNA Microarrays: A Practical Approach is ideal for researchers investigating patterns of gene expression (and the relationship with disease), and it is essential for any researcher interested in the use of biochips.

'...With its well-laid out protocols, the book manages to instruct the reader well and should be accessible to both novice and expert molecular biologists alike' Aslib Book Guide '...it's an essential purchase and probably the cheapest one a microarraying lab will make' Microbiology Today

1: Mark Schena and Ronald W. Davis: Genes, genomes, and chips: Introduction; Information content of genomes; microarray analysis; Applications of microarrays; Chips and pharmacogenomics; Summary; References 2: Mack J. Schermer: Confocal scanning microscopy in microarray detection: Introduction - microarrays, fluorescence, and detection; Sample handling; Confocal scanning of microarrays; Wavelength discrimination - minimizing the background image; Detectors; Signal processing and instrument control; Instrument performance measures; Scanarray (registered trademark) confoccal microarray scanners; References 3: Stanley F. Nelson and Christopher T. Denny: Representational differences analysis and microarray hybridization for efficient cloning and screening of differentially expressed genes: Introduction; RDA; Generation of libraries from RDA subtractions; Microarray fabrication; microarray hybridization; Differential expression screening with microarrays; Summary; References 4: Stephen Case-Green, Clare Pritchard, and Edwin M. Southern: Use of oligonucleotide arrays in enzymatic assays: assay optimization: Introduction; Fabrication of arrays; Preparation of target DNA; Hybridization to arrays; Enzyme catalysed extension reactions; Variations on protocols; References 5: John K. Elder, Martin Johnson, Natalie Milner, Kalim U. Mir, Muhammad Sohail, and Edwin M. Southern: Antisense oligonucleotide scanning arrays: Introduction; Array fabrication; Acknowledgements; References 6: Thomas P. Theriault, Scott C, Winder, and Ronald C, Gamble: Application of ink-jet printing technology to the manufacture of molecular arrays: Introduction; Ink-jet technology; Molecular array fabrication by synthesis; Molecular array fabrication by deposition; Conclusion; Acknowledgements; References 7: Ranelle C. Salunga, Hongqing Guo, Lin Luo, Anton Bittner, K. C. Joy, Jim R. Chambers, Jackson S. Wan, Michael R. Jackson, and Mark G. Erlander: Gene expression analysis via cDNA microarrays of laser capture microdissected cells from fixed tissue: Ranelle C. Salunga, Hongqing Guo, Lin Luo, Anton Bittner, K. C. Joy, Jim R. Chambers, Jackson S. Wan, Michael R. Jackson, and Mark G. Erlander: Introduction; Laser capture microdissection; Generation of aRNA - first round; cDNA microarrays; Acknowledgements; References 8: Joel Lloyd Bellenson: Expression data and the bioinformatics challenges: Genes/proteins; The raw data pipeline; The reference data warehouse; The structure of molecular information; Medial lexicon and donor sample registration; Data mining an expression data mart; Conclusions; References 9: Michael J. Heller, Eugene Tu, Anita Holmsen, Ronald G. Sosnowski, and James O'Connell: Active microelectronic arrays for DNA hybridization analysis: Introduction; Background on electronic array and hybridization technology; Active microelectronics array fabrication; Point mutation analysis on active electronic arrays; Microfabrication of active DNA chips; Example of single base discrimination analysis; Conclusions; References 10: Renu A. Heller, John Allard, Fengrong Zuo, Christopher Lock, Stacy Wilson, Paul Klonowski, Hans Gmuender, Harold Van Wart, and Robert Booth: Gene chips and microarrays: applications in disease profiles, drug target discovery, drug action and toxicity: Introduction; Methods; Results; Discussion; Acknowledgements; References Appendix Index

ISBN: 9780199637768
ISBN-10: 0199637768
Series: Practical Approach Series
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 232
Published: 1st August 1999
Publisher: Oxford University Press
Country of Publication: GB
Dimensions (cm): 24.13 x 15.88  x 1.27
Weight (kg): 0.41