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Diagnostic Virology Protocols : Methods in Molecular Biology - John R. Stephenson

Diagnostic Virology Protocols

Methods in Molecular Biology

By: John R. Stephenson (Editor), Alan Warnes (Editor)

Hardcover Published: 2nd December 2010
ISBN: 9781607618164
Number Of Pages: 470

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Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

From the reviews of the second edition:

"In its 23 chapters it covers most ... viruses of interest to the diagnostic virologist. Each chapter is written by an authority in the field and is composed of a short introduction followed by a number of protocols. These are sufficiently detailed to allow the reader to set up the assays in their own lab. ... Most chapters address a virus type, but vary such that in some the introduction is very informative whilst in others it is merely a lead into the protocols." (Dave Yirrell, Microbiology Today, November, 2011)

Prefacep. v
Contributorsp. xi
Detection of Adenovirusesp. 1
Alphavirusesp. 17
Detection of Human Caliciviruses in Fecal Samples by RT-PCRp. 39
Detection of Dengue Virusp. 51
Detection of Enteroviruses from Clinical Specimensp. 65
The Detection of Hepatitis Virusesp. 79
Molecular Detection of Herpesvirusesp. 101
HIV Drug Resistance Testingp. 123
Detection, Quantification, and Characterisation of HIV/SIVp. 133
Simultaneous Molecular Detection and Confirmation of Influenza AH5, with Internal Controlp. 161
Detection of Measles, Mumps, and Rubella Virusesp. 183
Detection of High-Risk Mucosal Human Papillomavirus DNA in Human Specimens by a Novel and Sensitive Multiplex PCR Method Combined with DNA Microarrayp. 195
The Detection of Parvovirusesp. 213
Detection and Characterization of Poliovirusesp. 233
Detection of Human-Pathogenic Poxvirusesp. 257
Lyssaviruses: Special Emphasis on Rabies Virus and Other Members of the Lyssavirus Genusp. 279
Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogensp. 309
Rotavirusesp. 325
Detection of Crimean-Congo Hemorrhagic Fever, Hanta, and Sandfly Fever Viruses by Real-Time RT-PCRp. 357
Detection of SARS Coronavirusp. 369
Detection of West Nile Virusp. 383
Point of Care Testing: Diagnosis Outside the Virology Laboratoryp. 415
Modelling Emerging Viral Epidemics for Public Health Protectionp. 435
Indexp. 467
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9781607618164
ISBN-10: 1607618168
Series: Methods in Molecular Biology : Book 665
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 470
Published: 2nd December 2010
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 25.4 x 17.8  x 3.18
Weight (kg): 1.04
Edition Number: 2
Edition Type: Revised