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Culture of Hematopoietic Cells : Culture of Specialized Cells - R. Ian Freshney

Culture of Hematopoietic Cells

Culture of Specialized Cells

Paperback

Published: August 1994
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Provides scientists with step-by-step methods for easy use in the lab when cultivating and experimenting with blood cells. Covering a range of human and mouse cell types, this laboratory manual includes contributions from more than twenty leading investigators who offer their practical expertise on a wide range of topics. Features detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.

Partial table of contents:

PRIMITIVE PROGENITOR ASSAYS.

Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher).

Assay for Murine Blast Cell Colonies (N. Katayama & M. Ogawa).

Serum-Deprived Cultures of Primary Hematopoietic Cells (G. Migliaccio & A. Migliaccio).

COLONY-FORMING ASSAYS FOR MALIGNANT CELLS.

Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, et al.).

LONG-TERM ASSAYS AND IMMORTALIZATION.

Long-Term Culture of Human Myeloid Cells (H. Sutherland & C. Eaves).

Immortalization of Hematopoietic Cells (C. MacDonald).

PURIFICATION OF PROGENITOR CELLS.

Purification of Murine Granulocyte-Macrophage Progenitor Cells (CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper & H. Broxmeyer).

OTHER SYSTEMS AND ASSAYS.

Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins & R. Fleischman).

List of Suppliers.

Index.

ISBN: 9780471588306
ISBN-10: 047158830X
Series: Culture of Specialized Cells
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 296
Published: August 1994
Country of Publication: US
Dimensions (cm): 25.4 x 17.9  x 1.5
Weight (kg): 0.73
Edition Number: 1