Techniques in Analytical Chemistry<br> How to evaluate and validate data from chemical measurements in biological systems-a unique overview<br> An understanding of chemical measurement processes in biological systems is essential to working in today's life-science and biotechnology industries. This work provides analysts and laboratory professionals with the fundamental knowledge and basic principles they need to tackle the measurement process in the real world. Rather than rehashing traditional analytical techniques, which can be problematic for biological samples, the book emphasizes the highly complex nature of biological systems through such topics as enzyme measurements, enzymes as reagents, immunochemical assays, and assay validation and quality control. It provides a complete overview of principles and methods proven useful in biological systems-from pH, buffers, and spectrophotometry to basic chromatographic techniques. With Chemical Measurements in Biological Systems as their guide, readers will understand the basic principles needed to:<br> * Read and evaluate an assay method description<br> * Choose the appropriate method from the vast array of available techniques<br> * Modify and evaluate an existing method for specific needs<br> * Set up and run biochemical assays<br> * Use multistep problem-solving techniques<br> * Evaluate the results of a given assay in a given matrix
"...Stewart and Ebel offer a textbook for graduate or advanced
undergraduate students in life sciences and a reference for
researchers. They introduce the fundamentals of making chemical
measurements..." (SciTech Book News, Vol. 24, No. 4, December
"In general, this is a book, which could be of enormous utility for
people working in biochemistry." (The Analyst, 8th October,
"there was a lot here that I liked and I found myself nodding in
agreement" (Chromatographia, November 2001)
"The style is readable and didactic and I think it succeeds as a
basic primer." (Chromatographia)
Water, pH, and Buffers.
Ultraviolet and Visible Absorption Spectrophotometry and
Detection Reactions (Colorimetric Reactions).
Enzymes and the Use of Enzymes as Reagents.
Measurement of Enzyme Activities.
Chromatography as a Tool for Measurement of Analytes in Complex
Electrophoresis and Other Electrokinetic Separations.
Enzyme-Linked Immunosorbant Assays.
Assay Quality Control and Data Validation.