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Cell Cycle Control : Volume 283 - John N. Abelson

Cell Cycle Control

Volume 283

By: John N. Abelson (Editor), Melvin I. Simon (Editor), William G. Dunphy (Editor)

Hardcover

Published: 25th July 1997
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General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features
* Cell cycle regulators in mammalian systems
* Cell cycle control in yeast and fungal systems
* Analysis of cell cycle regulators in oocyte, egg, and embryonic systems, as well as general methods

Praise for the Volume "This book will be invaluable to those studying the cell cycle, especially to students gaining experience in the techniques common to this field. The chapters have been written by those who are active in the field and have practical knowledge of the techniques involved. I would highly recommend the addition of this book to library and bookstores." --Ada A. Cole, Rush Medical College of Rush University, for DOODY'S PUBLISHING REVIEWSPraise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

"Incomparably useful." --ANALYTICAL BIOCHEMISTRY

"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY

"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

General Methodologies
Purification and Reconstitution of Cyclin-Dependent Kinase 2 in Four States of Activity
Purification of Recombinant Cyclin B1/Cdc2 Kinase from Xenopus Egg Extracts
Protein Phosphorylation Analysis by Electrospray Ionization Mass Spectrometry
Cloning Cell Cycle Regulatory Genes by Transcomplementation in Yeast
Fluorescence-Activated Cell Sorting of Transfected Cells
Microinjection of Antibodies into Mammalian Cells
Small Pool Expression Screening: Identification of Genes Involved in Cell Cycle Control, Apoptosis, and Early Development
Localization of Cell Cycle Regulators by Immunofluorescence
Chemical Inhibitors of Cyclin-Dependent Kinases
Identifying Human Homologs of Cell Cycle Genes using dbEST and XREFdb
Gene Identification Using the Yeast Two-Hybrid System
Cell Cycle Regulators in Mammalian Systems
Use of Tetracycline-Controlled Gene Expression Systems to Study Mammalian Cell Cycle
Regulation of Mammalian Cyclin-Dependent Kinase 2
Assay for Activity of Mammalian Cyclin D-Dependent Kinases CDK4 and CDK6
Functional Analysis of E2F Transcription Factor
Analysis of Mammalian Cdc37, A Protein Kinase Targeting Subunit of Heat Shock Protein 90
Purification and Analysis of CIP/KIP Proteins
Functional Dissection of the p53 Tumor Suppressor Protein
Reconstitution of Mammalian CDK-Activating Kinase
Characterization of Mammalian NIMA-Related Kinases
Generation of Phosphorylated Cyclin-Dependent Kinase 2 and Functional Characterization of Thrionine-160-Specific Phosphatase KAP
Isolation and Characterization of Temperature-Sensitive Mammalian Cell Cycle Mutants
Cell Cycle Control in Yeast and Fungal Systems
Use of Conditional Promoters in Budding Yeast
Flow Cytometric Analysis of DNA Content in Budding Yeast
Factor Synchronization of Budding Yeast
Elutriation of Budding Yeast
Functional Analysis of FAR1 in Yeast
Cell-Free Ubiquitination of Cell Cycle Regulators in Budding Yeast Extracts
Genomic Footprinting of Budding Yeast Replication Origins during Cell Cycle
Characterization of Budding Yeast Cdc7 Protein Kinase
Analysis of Budding Yeast Protein Kinases Con
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121821845
ISBN-10: 0121821846
Series: Methods in Enzymology : Book 283
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 678
Published: 25th July 1997
Publisher: ACADEMIC PR INC
Country of Publication: US
Dimensions (cm): 22.91 x 15.19  x 3.81
Weight (kg): 1.12