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Cardiovascular Physiology in the Genetically Engineered Mouse : DEVELOPMENTS IN CARDIOVASCULAR MEDICINE - Brian D. Hoit

Cardiovascular Physiology in the Genetically Engineered Mouse


By: Brian D. Hoit (Editor), Richard A. Walsh (Editor)

Hardcover Published: 31st October 2001
ISBN: 9780792375364
Number Of Pages: 403

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The enormous advances in molecular biology and genetics coupled with the progress in instrumentation and surgical techniques have produced a voluminous and often bewildering quantity of data. The primary objective of a second edition of Cardiovascular Physiology in the Genetically Engineered Mouse is to interpret critically the literature and to provide a framework for the enormous amount of information in this burgeoning field. As in the first edition, the monograph serves as a practical guide for the investigator interested in the functional methods used to characterize the murine cardiovascular phenotype.
The monograph is organized into three parts. The first deals with principles of transgenesis and homologous recombination. The second part, which again is the largest, discusses the various techniques used to assess the cardiovascular mechanical, metabolic, and electrophysiologic phenotype. This section is organized in a hierarchical manner- i.e. from isolated myocyte to isolated heart to the intact, anesthetized and conscious mouse. The third part examines techniques used to evaluate murine smooth muscle function, genetic mouse models of hypertrophy and heart failure, and the methods to assess the cardiovascular phenotype in the developing mouse embryo. In addition, newer methods that `push the envelope', such as magnetic resonance imaging (MRI), positron emission tomography (PET), ultrasound biomicroscopy (UBM), and computed tomography (microCT) are discussed. Expanded and updated, each chapter is richly enhanced with original tables and figures, and in many cases, extensively rewritten when compared with the first edition.
An essential and enduring goal of this second edition is to continue to facilitate interactions between the basic science disciplines and help bridge the gap between molecular biology and physiology.

The Hierarchial Approach to Analysis of Cardiovascular Function in Mice: An Overviewp. 1
Targeted Ablation of Murine Cardiac Genesp. 17
Transgenic Models of Cardiovascular Physiology and Pathophysiologyp. 37
Isolated Myocyte Mechanics and Calcium Transientsp. 55
The Isolated Isovolumic (Langendorff) Heart Preparationp. 69
The Isolated Work-Performing Mouse Heart Preparation. Comparison and Quantification of Cardiac Performance in Transgenic and Wild-Type Micep. 77
Echocardiographic Assessment of the Mouse Heart and Aortap. 97
Invasive Hemodynamic Studies in Open and Closed Chest Micep. 111
Cardiovascular Physiology in Mice: Conscious Measurements and Effects of Anesthesiap. 125
Single Cell Patch-Clamp Analysis of Mouse Myocytesp. 145
In Vivo Electrophysiologic Studies in Micep. 161
[superscript 31]p NMR Spectroscopy of the Mouse Heartp. 183
Models of Cardiac Disease in the Mousep. 193
Exercise and Oxygen Consumption in the Mousep. 213
Mouse Smooth Muscle Studies Using Gene-Altered Mouse Models: A Users' Guidep. 247
Analysis of Murine Embryonic Structural and Functional Phenotypep. 259
Indexp. 281
Table of Contents provided by Blackwell. All Rights Reserved.

ISBN: 9780792375364
ISBN-10: 079237536X
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 403
Published: 31st October 2001
Publisher: Springer
Country of Publication: NL
Dimensions (cm): 24.77 x 17.15  x 2.54
Weight (kg): 0.73
Edition Number: 2
Edition Type: Revised