Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.
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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
* Preparation of substrates and assay of enzymes
* Cloning, expression, and purification of enzymes
* Detection and consequences of genetic defects
* Regulation and expression of enzymes
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Section I: Preparation of Substrates, Assays of Intermediates and Enzymes, and Use of Enzyme Inhibitors : Synthesis and Gas Chromatography/Mass Spectrometry Analysis of Stereoisomers of 2-Hydroxy-3-methylpentanoic Acid : Analysis of Intracellular Metabolites as Tool for Studying Branched-Chain Amino Acid Biosynthesis and Its Inhibition in Bacteria : Determination of Branched-Chain L-Amino-Acid Aminotransferase Activity : Analysis of (S)- and (R)-3-Methyl-2-oxopentanoate Enantiomorphs in Body Fluids : Spectrophotometric Assay for Measuring Branched-Chain Amino Acids : Determination of Branched-Chain alpha-Keto Acid Dehydrogenase Activity State and Branched-Chain alpha-Keto Acid Dehydrogenase Kinase Activity and Protein in Mammalian Tissues : Simultaneous Quantification of Plasma Levels of alpha-Ketoisocaproate and Leucine by Gas Chromatography-Mass Spectrometry : Synthesis of Methacrylyl-CoA and (R)- and (S)-3-Hydroxyisobutyryl-CoA : Pathways of Leucine and Valine Catabolism in Yeast Section II: Cloning, Expression, and Purification of Enzymes of Branched-Chain Amino Acid Metabolism : Isolation of Subunits of Acetohydroxy Acid Synthase Isozyme III and Reconstitution of Holoenzyme : Branched-Chain Amino-Acid Aminotransferase of Escherichia coli : Purification of Sodium-Coupled Branched-Chain Amino Acid Carrier of Pseudomonas aeruginosa : Reconstitution of Pseudomonas aeruginosa High-Affinity Branched-Chain Amino Acid Transport System : Purification of Pseudomonas putida Branched-Chain Keto Acid Dehydrogenase E1 Component : Pseudomonas mevalonii 3-Hydroxy-3-methylglutaryl-CoA Lyase : Human 3-Hydroxy-3-methylglutaryl-CoA Lyase : Branched-Chain alpha-Keto Acid Dehydrogenase Kinase : Expression of E1 Component of Human Branched-Chain alpha-Keto Acid Dehydrogenase Complex in Escherichia coli by Cotransformation with Chaperonins GroEL GroES : Production of Recombinant Mammalian Holo-E2 and E3 and Reconstitution of Functional Branched-Chain alpha-Keto Acid Dehydrogenase Complex with Recombinant E1 : Production of Recombinant E1 Component of Branched-Chain alpha-Keto Acid Dehydrogenase Complex : Mammalian Methylmalonate-Semialdehyde Dehydrogenase : Mammalian 3-Hydroxyisobutyrate Dehydrogenase : 3-Hydroxyisobutyryl-CoA Hydrolase : Mammalian Branched-Chain Acyl-CoA Dehydrogenases: Molecular Cloning and Characterization of Recombinant Enzymes : 3-Hydroxy-3-methylglutaryl-CoA Reductase : Characterization of 3-Methylcrotonyl-CoA Carboxylase from Plants : Purification of D-Hydroxyisovalerate Dehydrogenase from Fusarium sambucinum : Purification and Characterization of Recombinant 3-Isopropylmalate Dehydrogenases from Thermus thermophilus Other Microorganisms : Wild-Type and Hexahistidine-Tagged Derivatives of Leucine-Responsive Regulatory Protein from Escherichia coli : Purification of Branched-Chain Keto Acid Dehydrogenase Regulator from Pseudomonas putida : Mitochondrial Import of Mammalian Branched-Chain alpha-Keto Acid Dehydrogenase Complex Subunits : Cloning, Expression, and Purification of Mammalian 4-Hydroxyphenylpyruvate Dioxygenase/alpha-Ketoisocaproate Dioxygenase : Mammalian Branched-Chain Aminotransferases : Branched-Chain-Amino-Acid Transaminases of Yeast Saccharomyces cerevisiae : Purification, Properties, and Sequencing of Aminoisobutyrate Aminotransferases from Rat Liver : Branched-Chain Keto Acid Dehydrogenase of Yeast : ss-Alanine Synthase an Enzyme Involved in Catabolism of Uracil and Thymine Section III: Detection and Consequences of Genetic Defects in Genes Encoding Enzymes of Branched-Chain Amino Acid Metabolism : Diagnosis and Mutational Analysis of Maple Syrup Urine Disease Using Cell Cultures : Detection of Gene Defects in Branched-Chain Amino Acid Metabolism by Tandem Mass Spectrometry of Carnitine Esters Produced by Cultured Fibroblasts : Molecular and Enzymatic Methods for Detection of Genetic Defects in Distal Pathways of Branched-Chain Amino Acid Metabolism : Genetic Defects in E3 Component of alpha-Keto Acid Dehydrogenase Complexes : Targeting E3 Component of alpha-Keto Acid Dehydrogenase Complexes Section IV: Regulation and Expression of Enzymes of Branched-Chain Amino Acid Metabolism : Regulation of Expression of Branched-Chain alpha-Keto Acid Dehydrogenase Subunits in Permanent Cell Lines : Expression of Murine Branched-Chain alpha-Keto Acid Dehydrogenase Kinase : Regulation of Branched-Chain alpha-Keto Acid Dehydrogenase Kinase Gene Expression by Glucocorticoids in Hepatoma Cells and Rat Liver Author Index Subject Index
Series: Methods in Enzymology : Book 324
Number Of Pages: 550
Published: 8th September 2000
Publisher: ACADEMIC PR INC
Country of Publication: US
Dimensions (cm): 23.55 x 15.9
Weight (kg): 0.91