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Biophysical Labeling Methods in Molecular Biology - Gertz I. Likhtenshtein

Biophysical Labeling Methods in Molecular Biology

Hardcover Published: 26th March 1993
ISBN: 9780521431323
Number Of Pages: 320

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This monograph describes the theoretical bases and experimental prerequisites for methods such as spin fluorescence, triplet, Mossbauer, photochromic and electron-density labeling, including the procedures used to obtain specifically modified proteins, enzymes, biomembranes, nucleic acids, and other biological molecules. The fundamentals of the physical theory behind each technique is explained and details are given of how to interpret the experimental data obtained. Special sections deal with critical reviews of recent data on the structure, molecular dynamics and conformational transitions of biological molecules. Each section concludes with a discussion of the results obtained from these techniques in connection with various problems of enzyme catalysis, electron transfer, molecular biophysics and molecular biology. The uses that labeling techniques can be put to for the investigation of whole cells and tissues are also discussed.

"Labeling methods, including fluorescence and spin labeling, are widely used to probe the structure and function of biomolecules...I strongly encourage anyone who wants an intense and well structured introduction into the field of labeling to read Likhenshtein's book. Even the experienced researcher will benefit from it because of the parallel presentation of several labeling methods." Klaus Gawrisch, Analytical Biochemistry "...should be of use to graduate students and those considering research in biophysical chemistry who want an historical overview of labeling methods." Bruce Eaton, Journal of the American Chemical Society

The methods of spin labeling
Double labeling techniques
Fluorescent labeling methods
Triplet labeling methods
Mossbauer, electron-scattering, and other labeling methods
Studies of proteins and enzymes: structure, dynamics and mechanism of action
Structure and dynamics of membrane
Nucleic acids and other biosystems, biological assays
Conclusion
References
Subject index
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780521431323
ISBN-10: 0521431328
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 320
Published: 26th March 1993
Publisher: Cambridge University Press
Country of Publication: GB
Dimensions (cm): 24.13 x 16.51  x 1.91
Weight (kg): 0.59