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Biomembranes : Biomembranes, Part P: ATP-Driven Pumps and Related Transport: The Na,K-Pump ATP-Driven Pumps and Related Transport: the Na,K-Pump: Part P Volume 156 - John N. Abelson

Biomembranes

Biomembranes, Part P: ATP-Driven Pumps and Related Transport: The Na,K-Pump ATP-Driven Pumps and Related Transport: the Na,K-Pump: Part P Volume 156

By: John N. Abelson (Editor), Melvin I. Simon (Editor), Sidney Fleischer (Editor), Becca Fleischer (Editor)

Hardcover

Published: 28th March 1988
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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Overview: The Na,K-Pump. Preparation of Na+,K+-ATPase and Subunits
Purification of Na+,K+-ATPase: Enzyme Sources, Preparative Problems, and Preparation from Mammalian Kidney
Preparation of Membrane Na+,K+-ATPase from Rectal Glands of Squalus acanthias
Purification of Na+,K+-ATPase from the Supraorbital Salt Gland of the Duck
Preparation of Na+,K+-ATPase from Brine Shrimp
Preparation of the *ga(+) Isozyme of the Na+,K+-ATPase from Mammalian Axolemma
Solubilization of Na+,K+-ATPase
Crystallization of Membrane-Bound Na+,K+-ATPase in Two Dimensions
Preparation of Antibodies to Na+,K+-ATPase and Its Subunits
Assay of Na+,K+-ATPase Activity
ATPase and Phosphatase Activity of Na+,K+-ATPase: Molar and Specific Activity, Protein Determination
Coupled Assay of Na+,K+-ATPase Activity
Identification and Quantitation of Na+,K+-ATPase by Back-Door Phosphorylation
Reconstitution of Na,K-Pump Activity
Reconstitution of Na,K-Pump Activity by Cholate Dialysis: Sidedness and Stoichiometry
Reconstitution of the Na,K-Pump by Freeze*b1Thaw Sonication: Estimation of Coupling Ratio and Electrogenicity
Incorporation of C12E8-Solubilized Na+,K+-ATPase into Liposomes: Determination of Sidedness and Orientation
Analysis of the Pump Cycle
Measurement of Na+ and K+ Transport and Na+,K+-ATPase Activity in Inside-Out Vesicles from Mammalian Erythrocytes
Measurement of Active and Passive Na+ and K+ Fluxes in Reconstituted Vesicles
Measurement of Ligand Binding and Distance between Ligands
Measurement of Binding of ATP and ADP to Na+,K+-ATPase
Interaction of Cardiac Glycosides with Na+,K+-ATPase
Estimation of Na,K-Pump Numbers and Turnover in Intact Cells with [3H]Ouabain
Measurement of Binding of Na+, K+, and Rb+ to Na+,K+-ATPase by Centrifugation Methods
Estimating Affinities for Physiological Ligands and Inhibitors by Kinetic Studies on Na+,K+-ATPase and Its Partial Activities
Inhibition of Translocation Reactions by Vanadate
Measurements of Conformational States of Na+,K+-ATPase
Use of Formycin Nucleotides, Intrinsic Protein Fluorescence, and Fluorescein Isothiocyanate-Labeled Enzymes for Measurement of Conformational States of Na+,K+-ATPase
Eosin as a Fluorescence Probe for Measurement of Conformational States of Na+,K+-ATPase
Rapid Ion-Exchange Technique for Measuring Rates of Release of Occluded Ions
Modification of Na+,K+-ATPase
Proteolytic Cleavage as a Tool for Studying Structure and Conformation of Pure Membrane-Bound Na+,K+-ATPase
Irreversible and Reversible Modification of SH Groups and Effect on Catalytic Activity
Photoaffinity Labeling with ATP Analogs
Affinity Labeling of the Digitalis-Binding Site
Determination of Quaternary Structure of an Active Enzyme Using Chemical Cross-Linking with Glutaraldehyde
Use of Cross-Linking Reagents for Detection of Subunit Interactions of Membrane-Bound Na+,K+-ATPase
Magnetic Resonance Studies of Na+,K+-ATPase
Nuclear Magnetic Resonance Investigations of Na+,K+-ATPase
Electron Spin Resonance Investigations of Na+,K+-ATPase. Biogenesis and Membrane Assembly
Molecular Cloning of Na+,K+-ATPase *ga Subunit Gene Usin
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780121820572
ISBN-10: 0121820572
Series: Methods in Enzymology : Book 156
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 468
Published: 28th March 1988
Publisher: ACADEMIC PR INC
Country of Publication: US
Dimensions (cm): 22.91 x 15.19  x 2.69
Weight (kg): 0.84