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Biofilms : Investigative Methods and Applications - Hans-Curt Flemming


Investigative Methods and Applications

Hardcover Published: 23rd June 2000
ISBN: 9781566768696
Number Of Pages: 268

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Until now, techniques for studying biofilms- the cellular colonies that live in drinking water systems, wastewater operations, even ground and surface water- have been limited. Yet during the last decade, biofilms have become a critical element in water quality preservation systems, a key component of wastewater treatment biological reactions and the subject of extensive microbiological inquiry. An understanding of biofilm development, structure and dynamics is one condition for improving water supplies and for addressing technical problems such as biofouling, corrosion and bioweathering.

Biofilms: Investigative Methods and Applications provides the first in-depth assessment of current and experimental ways to study biofilms, both in sample form and in situ. It shows how sensors, microscopy, lasers, and calorimetry, among other tools, can be used to obtain data on the morphology and metabolism of biofilms. This text is the first to organize and examine the best methods for investigating biofilms. It covers culture-based methods and emerging nondestructive techniques. It also shows how they can be used to characterize biofilms in a variety of manmade settings, such as sewers, wastewater plants, and drinking water distribution systems, as well as in karsts and groundwater sources.

In clarifying the way biofilms are studied, the book offers new insights into these living films. It also applies inquiry techniques to the many problems confronting the environmental specialist-corrosion control, biofouling, and the improvement of fixed reactors in wastewater treatment. At the same time it explains technologies for the controlled growth of biofilms and shows how biofilms can be effectively monitored and subjected to quantitative analysis. The technical information in Biofilms: Investigative Methods and Applications is designed to be of use to engineers and researchers and to be helpful in the generation of electronic data.

Prefacep. xi
List of Contributorsp. xiii
Steps in Biofilm Sampling and Characterization in Biofouling Casesp. 1
Introductionp. 1
Sampling Strategyp. 2
Field Methods In Situp. 3
Laboratory Methodsp. 4
Laboratory Methods to Examine Removed Biofilmsp. 10
Identificationp. 11
Chemical and Biochemical Analysisp. 13
Genetic Methodsp. 16
Immunological Methodsp. 17
Conclusions and Outlookp. 17
Referencesp. 18
Rotating Annular Reactors for Controlled Growth of Biofilmsp. 23
Introductionp. 23
Material and Methodsp. 25
Resultsp. 29
Discussionp. 34
Referencesp. 39
Microcalorimetry for Evaluating Countermeasures Against Biofouling in Water Circulation Systemsp. 41
Introductionp. 41
Materials and Methodsp. 42
Results and Discussionp. 44
Conclusionsp. 48
Acknowledgementp. 49
Referencesp. 49
Extraction and Quantification of Extracellular Polymeric Substances From Wastewaterp. 51
Introductionp. 51
Extraction Methodsp. 53
Quantificationp. 57
Cell Lysisp. 59
Applicationsp. 60
Acknowledgementsp. 64
Nomenclaturep. 64
Referencesp. 65
Cell Biomass Determination in Sewer Biofilms and a Monospecies Pseudomonas Putida Biofilmp. 69
Introductionp. 69
Direct Determinationp. 71
Indirect Methodsp. 77
Conclusionsp. 80
Referencesp. 81
Simultaneous Morphological and Population Analyses of Environmental Biofilmsp. 83
Principle of the Methodp. 83
Range of Applicationsp. 84
Methodsp. 85
Resultsp. 94
Comparison with Other Methodsp. 98
Discussionp. 100
Nomenclaturep. 103
Acknowledgementsp. 104
Referencesp. 104
Development of a New Method to Determine the Metabolic Potential of Bacteria in Drinking Water Biofilms: Probe Active Counts (Pac)p. 107
Introductionp. 107
Materials and Methodsp. 108
Results and Discussionp. 111
Referencesp. 119
Formation and Bacterial Composition of Young, Natural Biofilms Obtained From Public Bank-Filtered Drinking Water Systemsp. 123
Introductionp. 123
Materials and Methodsp. 124
Resultsp. 128
Discussionp. 136
Acknowledgementsp. 140
Referencesp. 140
The Use of Immunological Techniques and Scanning Confocal Laser Microscopy for the Characterization of Agrobacterium Tumefaciens and Pseudomonas Fluorescens, Atrazine-Utilizing Biofilmsp. 143
Introductionp. 143
Materials and Methodsp. 144
Resultsp. 147
Discussionp. 151
Acknowledgementsp. 152
Referencesp. 152
The in Situ Detection of a Microbial Biofilm Community on Karst Rock Coupon in a Groundwater Habitatp. 155
Introductionp. 155
Materials and Methodsp. 156
Results and Discussionp. 157
Summaryp. 161
Referencesp. 162
Investigation of Spatial and Temporal Gradients in Fixed-Bed Biofilm Reactors for Wastewater Treamentp. 165
Introductionp. 165
Aim of the Studyp. 166
Experimental Setupp. 167
Chlorophenol Elimination by Biofilms Under Different Operating Conditionsp. 168
Quantitative Determination of Biomass and Biological Activityp. 171
Investigation of the Bacterial Community of the Biofilmp. 178
The Protozoa and Metazoa in Biofilmsp. 183
Conclusionsp. 188
Nomenclaturep. 190
Acknowledgementsp. 191
Referencesp. 191
Quantitative Microscopy in Biofilm Studiesp. 195
Introductionp. 195
Materials and Methodsp. 195
Resultsp. 198
Discussionp. 208
Acknowledgementsp. 208
Referencesp. 208
Confocal Laser Scanning Microscopy (Clsm) of Biofilmsp. 211
Introductionp. 211
Clsm Historyp. 212
Confocal Imagingp. 213
Advantages/Disadvantagesp. 213
Techniquesp. 214
Applicationsp. 217
Conclusionsp. 219
Acknowledgementp. 219
Referencesp. 219
Microelectrodes and Tube Reactors in Biofilm Researchp. 225
Introductionp. 225
Materials and Methodsp. 226
Resultsp. 231
Discussionp. 238
Referencesp. 239
Indexp. 241
Table of Contents provided by Syndetics. All Rights Reserved.

ISBN: 9781566768696
ISBN-10: 1566768691
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 268
Published: 23rd June 2000
Country of Publication: US
Dimensions (cm): 24.03 x 15.29  x 2.41
Weight (kg): 0.6
Edition Number: 1

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