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Bioengineering and Molecular Biology of Plant Pathways : Volume 1 - Hans Bohnert

Bioengineering and Molecular Biology of Plant Pathways

Volume 1

Hardcover

Published: 17th December 2007
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The increased knowledge about the structure of genomes in a number of species, about the complexity of transcriptomes, and the rapid growth in knowledge about mutant phenotypes have set off the large scale use of transgenes to answer basic biological questions, and to generate new crops and novel products. This volume includes twelve chapters, which to variable degrees describe the use of transgenic plants to explore possibilities and approaches for the modification of plant metabolism, adaptation or development. The interests of the authors range from tool development, to basic biochemical know-how about the engineering of enzymes, to exploring avenues for the modification of complex multigenic pathways, and include several examples for the engineering of specific pathways in different organs and developmental stages.
* Prologue by Paul K. Stumpf and Eric E. Conn
* Incorporates new concepts and insights in plant biochemistry and biology
* Provides a conceptual framework regarding the challenges faced in engineering pathways
* Discusses potential in engineering of metabolic end-products that are of vast economical importance including genetic engineering of cellulose, seed storage proteins, and edible and industrial oils

Contributorsp. xi
Introduction to the Series and Acknowledgementsp. xv
Preface to Volume 1p. xvii
Prologuep. xxi
Metabolic Organization in Plants: A Challenge for the Metabolic Engineerp. 1
Introductionp. 2
Plant Metabolic Networks and Their Organizationp. 3
Tools for Analyzing Network Structure and Performancep. 7
Integration of Plant Metabolismp. 15
Summaryp. 22
Acknowledgementsp. 23
Referencesp. 23
Enzyme Engineeringp. 29
Introductionp. 30
Theoretical Considerationsp. 31
Practical Considerations for Engineering Enzymesp. 35
Opportunities for Plant Improvement Through Engineered Enzymes and Proteinsp. 42
Summaryp. 44
Acknowledgementsp. 44
Referencesp. 44
Genetic Engineering of Amino Acid Metabolism in Plantsp. 49
Introductionp. 51
Glutamine, Glutamate, Aspartate, and Asparagine are Central Regulators of Nitrogen Assimilation, Metabolism, and Transportp. 52
The Aspartate Family Pathway that is Responsible for Synthesis of the Essential Amino Acids Lysine, Threonine, Methionine, and Isoleucinep. 60
Regulation of Methionine Biosynthesisp. 66
Engineering Amino Acid Metabolism to Improve the Nutritional Quality of Plants for Nonruminants and Ruminantsp. 69
Future Prospectsp. 73
Summaryp. 74
Acknowledgementsp. 74
Referencesp. 74
Engineering Photosynthetic Pathwaysp. 81
Introductionp. 82
Identification of Limiting Steps in the PCR Cyclep. 83
Engineering CO[subscript 2]-Fixation Enzymesp. 85
Engineering Post-RuBisCO Reactionsp. 95
Summaryp. 97
Acknowledgementsp. 98
Referencesp. 99
Genetic Engineering of Seed Storage Proteinsp. 107
Introductionp. 108
Storage Protein Modification for the Improvement of Seed Protein Qualityp. 113
Use of Seed Storage Proteins for Protein Quality Improvements in Nonseed Cropsp. 119
Modification of Grain Biophysical Propertiesp. 120
Transgenic Modifications that Enhance the Utility of Seed Storage Proteinsp. 122
Summary and Future Prospectsp. 124
Acknowledgementsp. 127
Referencesp. 127
Biochemistry and Molecular Biology of Cellulose Biosynthesis in Plants: Prospects for Genetic Engineeringp. 135
Introductionp. 136
The Many Forms of Cellulose-A Brief Introduction to the Structure and Different Crystalline Forms of Cellulosep. 137
Biochemistry of Cellulose Biosynthesis in Plantsp. 139
Molecular Biology of Cellulose Biosynthesis in Plantsp. 144
Mechanism of Cellulose Synthesisp. 151
Prospects for Genetic Engineering of Cellulose Biosynthesis in Plantsp. 152
Summaryp. 154
Acknowledgementsp. 155
Referencesp. 155
Metabolic Engineering of the Content and Fatty Acid Composition of Vegetable Oilsp. 161
Introductionp. 163
TAG Synthesisp. 167
Control of TAG Compositionp. 175
Summaryp. 189
Acknowledgementsp. 192
Referencesp. 192
Pathways for the Synthesis of Polyesters in Plants: Cutin, Suberin, and Polyhydroxyalkanoatesp. 201
Introductionp. 202
Cutin and Suberinp. 203
Polyhydroxyalkanoatep. 213
Referencesp. 232
Plant Sterol Methyltransferases: Phytosterolomic Analysis, Enzymology, and Bioengineering Strategiesp. 241
Introductionp. 242
Pathways of Phytosterol Biosynthesisp. 244
Phytosterolomicsp. 251
Enzymology and Evolution of the SMTp. 258
Bioengineering Strategies for Generating Plants with Modified Sterol Compositionsp. 268
Acknowledgementsp. 276
Referencesp. 276
Engineering Plant Alkaloid Biosynthetic Pathways: Progress and Prospectsp. 283
Introductionp. 284
Monoterpenoid Indole Alkaloidsp. 286
Tetrahydrobenzylisoquinoline Alkaloidsp. 292
Tropane Alkaloidsp. 299
Summaryp. 304
Acknowledgementsp. 305
Referencesp. 305
Engineering Formation of Medicinal Compounds in Cell Culturesp. 311
Introductionp. 312
Biochemistry and Cell Biology of Secondary Metabolitesp. 314
Cell Culture and Metabolite Productionp. 325
Beyond the Obstacles: Molecular Biological Approaches to Improve Productivity of Secondary Metabolites in Plant Cellsp. 331
Future Perspectivesp. 337
Summaryp. 338
Acknowledgementsp. 338
Referencesp. 338
Genetic Engineering for Salinity Stress Tolerancep. 347
Salinity Stress Engineeringp. 348
The Context of Salinity Stressp. 349
Ion Homeostasisp. 353
Strategies to Improve Salt Tolerance by Modulating Ion Homeostasisp. 358
Strategies to Improve Salt Tolerance by Modulating Metabolic Adjustmentsp. 359
Plant Signal Transduction for Adaptation to Salinityp. 369
ABA is a Major Mediator of Plant Stress Response Signalingp. 371
Summaryp. 373
Acknowledgementsp. 374
Referencesp. 374
Metabolic Engineering of Plant Allyl/Propenyl Phenol and Lignin Pathways: Future Potential for Biofuels/Bioenergy, Polymer Intermediates, and Specialty Chemicals?p. 385
Introductionp. 387
Lignin Formation and Manipulationp. 389
Current Sources/Markets for Specialty Allyl/Propenyl Phenolsp. 404
Biosynthesis of Allyl and Propenyl Phenols and Related Phenylpropanoid Moietiesp. 406
Potential for Allyl/Propenyl Phenols?p. 415
Summaryp. 421
Acknowledgementsp. 421
Referencesp. 421
Author Indexp. 429
Subject Indexp. 445
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9780080449722
ISBN-10: 0080449727
Series: Advances in Plant Biochemistry and Molecular Biology
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 504
Published: 17th December 2007
Publisher: Elsevier Science & Technology
Country of Publication: GB
Dimensions (cm): 24.0 x 16.5  x 2.54
Weight (kg): 1.06
Edition Number: 1