Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology.ÿ In this second edition of Bioconjugation Protocols:ÿ Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering.ÿ These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches.ÿ Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices).ÿ All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition
offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.
Part I: Protein Conjugates 1. Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities Edward A. Lemke 2. Enzymatically-Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase Ahmed Besheer, Thomas C. Hertel, Jorg Kressler, Karsten Mader, and Markus Pietzsch 3. Synthesis of Drug/Dye-Incorporated Polymer-Protein Hybrids Sukanta Dolai, Wei Shi, Bikash Mondal, and Krishnaswami Raja 4. Dye/DNA Conjugates as Multiple Labels for Antibodies in Sensitive Fluorescence Immunoassays Qin Zhang, Shengchao Zhu, and Liang-Hong Guo 5. Chemoselective Modification of Viral Proteins Bearing Metabolically Introduced 'Clickable' Amino Acids and Sugars Partha S. Banerjee and Isaac S. Carrico 6. Preparation of Peptide and Other Biomolecular Conjugates through Chemoselective Ligations Mathieu Galibert, Olivier Renaudet, Didier Boturyn, and Pascal Dumy 7. New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed Covalent Protein Labeling Noriho Kamiya and Hiroki Abe 8. Covalent Conjugation of Poly(Ethylene Glycol) to Proteins and Peptides: Strategies And Methods Anna Mero, Chiara Clementi, Francesco M. Veronese, and Gianfranco Pasut 9. Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag to Proteins Using Protein Trans-Splicing Tulika Dhar, Thomas Kurpiers, and Henning D. Mootz Part II: Nucleic Acid Conjugates 10. Polyethylenimine Bioconjugates for Imaging and DNA Delivery In Vivo Andrea Masotti and Francesco Pampaloni 11. Synthesis of a Glycomimetic Oligonucleotide Conjugate By 1,3-Dipolar Cycloaddition Gwladys Pourceau, Albert Meyer, Jean-Jacques Vasseur, and Francois Morvan 12. Site-Specific DNA Labeling by Staudinger Ligation Samuel H. Weisbrod, Anna Baccaro, and Andreas Marx 13. Improved Cellular Uptake of Antisense Peptide Nucleic Acids (PNAs) by Conjugation to a Cell-Penetrating Peptide and a Lipid Domain Takehiko Shiraishi and Peter E. Nielsen 14. Synthesis of Oligonucleotide-Peptide Conjugates for Biomedical and Technological Applications Anna Avino, Santiago Grijalvo, Sonia Perez-Rentero, Alejandra Garibotti, Montserrat Terrazas, and Ramon Eritja 15. Amphiphilic DNA Block Copolymers: Nucleic Acid-Polymer Hybrid Materials for Diagnostics and Biomedicine Jan Zimmermann, Minseok Kwak, Andrew J. Musser, and Andreas Herrmann Part III: Glycosyl and Lipid Conjugates 16. Chemically Selective Liposome Surface Glyco-Functionalization Hailong Zhang, Yong Ma, and Xue-Long Sun 17. Bioconjugation Using Mutant Glycosyltransferases for the Site-Specific Labeling of Biomolecules with Sugars Carrying Chemical Handles Boopathy Ramakrishnan, Elizabeth Boeggeman, Marta Pasek, and Pradman K. Qasba 18. Lipid-Core-Peptide System for Self-Adjuvanting Synthetic Vaccine Delivery Mariusz Skwarczynski and Istvan Toth 19. Coupling Carbohydrates to Proteins for Glycoconjugate Vaccine Development Using a Pentenoyl Group as a Convenient Linker Qianli Wang and Zhongwu Guo 20. Conjugation of LPS-Derived Oligosaccharides to Proteins Using Oxime Chemistry Joanna Kubler-Kielb 21. Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast Junpeng Xiao and Thomas J. Tolbert 22. On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-linked Oligosaccharide Rui Chen and Thomas J. Tolbert 23. Design and Synthesis of Novel Functional Lipid-Based Bioconjugates for Drug Delivery and Other Applications Rupa R. Sawant and Vladimir P. Torchilin Part IV: Biofunctionalization of Surfaces and Thin Films 24. Chemical Functionalization and Bioconjugation Strategies for Atomic Force Microscope (AFM) Cantilevers Magnus Bergkvist and Nathaniel C. Cady 25. Chemoselective Protein and Peptide Immobilization on Biosensor Surfaces Edith H.M. Lempens, Brett A. Helms, and Maarten Merkx 26. Fabrication of Dynamic Self-Assembled Monolayers for Cell Migration and Adhesion Studies Nathan P. Westcott and Muhammad N. Yousaf 27. DNA Detection Using Functionalized Conducting Polymers Jadranka Travas-Sejdic, Hui Peng, Hsiao-hua Yu, and Shyh-Chyang Luo 28. Preparation and Dynamic Patterning Of Supported Lipid Membranes Mimicking Cell Membranes Stefan Kaufmann, Karthik Kumar, and Erik Reimhult 29. Enzyme Immobilization on Reactive Polymer Films Ana L. Cordeiro, Tilo Pompe, Mariana Tasso, Katrin Salchert, and Carsten Werner 30. Characterization of Protein--Membrane Binding Interactions Via a Microplate Assay Employing Whole Liposome Immobilization Matthew D. Smith and Michael D. Best 31. A Bioconjugated Phospholipid Polymer Biointerface with Nanometer-Scaled Structure for Highly Sensitive Immunoassays Kazuki Nishizawa, Madoka Takai, and Kazuhiko Ishihara Part V: Biofunctionalization of Nanostructures 32. Purification, Functionalization, and Bioconjugation of Carbon Nanotubes John H.T. Luong, Keith B. Male, Khaled A. Mahmoud, and Fwu-Shan Sheu 33. Functional Integration of Membrane Proteins with Nanotube and Nanowire Transistor Devices Aleksandr Noy, Alexander B. Artyukhin, Shih-Chieh Huang, Julio A. Martinez, and Nipun Misra 34. Single-Step Conjugation of Antibodies to Quantum Dots for Labeling Cell Surface Receptors in Mammalian Cells Gopal Iyer, Jianmin Xu, and Shimon Weiss 35. A Practical Strategy for Constructing Nanodrugs Using Carbon Nanotubes as Carriers Wei Wu and Xiqun Jiang 36. Design and Synthesis of Biofunctionalized Metallic/Magnetic Nanomaterials Eun-Kyung Lim, Seungjoo Haam, Kwangyeol Lee, and Yong-Min Huh
Series: Methods in Molecular Biology
Number Of Pages: 605
Published: 23rd June 2011
Publisher: Humana Press Inc.
Country of Publication: US
Dimensions (cm): 25.4 x 17.8
Weight (kg): 1.37
Edition Number: 2
Edition Type: Revised