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Automated DNA Sequencing and Analysis - Mark D. Adams

Automated DNA Sequencing and Analysis

By: Mark D. Adams (Editor), Chris Fields (Editor), J. Craig Venter (Editor)

Hardcover

Published: 9th March 2009
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A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects.
* The field of automation in DAN sequencing and analysis is rapidly moving. Hovever, as the technology becomes commonplace, those applying the techniques involved to their research fields need a text which both expands on the protocols supplied by manufacturers with their instruments and explains how to utilise the data produced. This book fulfils those needs, reviews the history of the art and provides pointers to future development.

Sequencing Instruments and Strategies
The Efficiency of Automated DNA Sequencing
Automated Multiplex Sequencing
Application of Capillary Array Electrophoresis to DNA Sequencing
Sequencing by Hybridization
Shotgun Sequencing
Shotgun Cloning as the Strategy of Choice to Generate Templates for High Throughput Dideoxynucleotide Sequencing
Transposon-Facilitated Large-Scale DNA Sequencing
Transposon-Facilitated Sequencing: An Effective Set of Procedures to Sequence DNA Fragments Smaller than 4kb
Construction of Exonuclease III Nested Deletion Sets for Rapid DNA Sequencing
Expressed Sequence Tags as Tools for Physiology and Genomics
The Use of Automated DNA Sequencing in the Analysis of cDNAs of Model Organisms
Sample Preparation and Sequencing Methods: Libraries
Applications of Cosmid Libraries in Genome Mapping and Sequencing Efforts
A Role for the P1 Cloning System in Genome Analysis
Generation and Mapping of Chromosome Specific Sequence-Tagged Sites (STS)
Construction of cDNA Libraries
Construction of Directionally Cloned cDNA Libraries and Phagemid Vectors
Construction of Directional cDNA Libraries
Preparation of cDNA Libraries from Brassica
Abundance Screening of Human cDNA Libraries
High Density Grid Technologies
Automated Sample Preparation
Solid Phrase Preparation of Sequencing Templates from PCR Products
Automatic Preparation of DNA Templatesfor Sequencing on the ABI Catalyst Robotic Workstation
Custom Magnetic Particles: Their Use in DNA Purification
Sequencing Methods
Large-Scale Automated Sequencing of Human Chromosomal Regions
Zen and the Art of Large-Scale Genomic Sequencing
Automated Dye Terminator DNA Sequencing
PCR Based Strategies for Gap Closure in Large Scale Sequencing Projects
Sequence Map Gaps and Directed Reverse Sequencing for the Completion of Large Sequencing Projects
Optimized Methods for Large-Scale Shotgun DNASequencing in Alu-Rich Genomic Regions
Use of Dimethylsulfoxide to Improve Fluorescent, Tag Cycle
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780127170107
ISBN-10: 0127170103
Audience: Professional
Format: Hardcover
Language: English
Number Of Pages: 368
Published: 9th March 2009
Publisher: Elsevier Science Publishing Co Inc
Country of Publication: US
Dimensions (cm): 22.9 x 15.2  x 2.54
Weight (kg): 0.9