| Preface | p. xv |
| Symposium | |
| Approaches to Human Gene Therapy for Next Century | |
| Improvement of adenovirus vectors for gene transfer | p. 1 |
| Gene therapy for genetic disease and cancer | p. 7 |
| Application of Chip Technology in Molecular and Cellular Biology | |
| Gene expression profiling of mouse postnatal cerebellar development Adaptor-tagged competitive PCR and its application to the mammalian nervous system | p. 13 |
| Mesenchymal Stem Cells for Reconstructive Surgery | |
| Generation of regenerated cardiomyocytes and its therapeutic use for cell transplantation into failing heart | p. 19 |
| Advanced Functional Foods and Water for Prevention of Diseases | |
| Reduced water for prevention of diseases | p. 25 |
| Clinical applications of electrolyzed-reduced water | p. 31 |
| Molecular Biology of Fish Cells in vitro | |
| Identification and characterization of fibronectin isoforms from zebrafish | p. 37 |
| Neurogenesis in zebrafish embryo cell cultures | p. 43 |
| Search for Bioactive Substances Using Animal Cells (Satellite Symposium Karatsu) | |
| Assay systems for screening food factors that have anti-proliferative and anti-invasive potential using rat ascites hepatoma cells in culture | p. 51 |
| Search for chemopreventive agents on the basis of the involvement of oxidative stress and inflammation in carcinogenesis | p. 57 |
| Functional analysis of sphingolipids using a specific enzyme and animal cells | p. 63 |
| Session | |
| Cell Culture Engineering | |
| Cell cultures and reverse transcriptase activity from a tumor of a moray eel | p. 67 |
| A simplified general artificial neural network for the modelization of culture kinetics of different CHO strains | p. 73 |
| Cell growth on modified polystyrene surface/ adhesion and detachment properties | p. 77 |
| Whole cell-smart microsensor: a first approach towards interfacing live cells and electronics | p. 81 |
| Copolymer of amino-acid and urethane as an artificial extracellular matrix to enhance the primary porcine hepatocytes attachment, proliferation and function | p. 85 |
| Primary culture of rat hepatocytes on an oxygen permeable membrane | p. 91 |
| Hydroxyapatite-pulp composite fiber sheet bed: a new material for the continuous culture of CHO-K1 cells | p. 97 |
| Relationship between cell cycle phases and monoclonal antibody production in microcarrier perfusion culture of recombinant CHO cells | p. 103 |
| Influence of bcl-2 over-expression on cell growth and antibody productivity of NS0 cells in perfusion and chemostat cultures | p. 109 |
| Dialysis perfusion culture of animal cells for the production of biologicals | p. 115 |
| Characterization of growth properties through morphological observation in culture of anchorage-dependent cells | p. 121 |
| Application of hepatocytes isolated from slaughtered pig for bioartificial liver support system | p. 127 |
| Culture of hepatocytes using non-woven PTFE fabric coated with copolymer of poly(amino acid) urethane | p. 133 |
| Production of Biologicals | |
| Hypoxia-induced production of recombinant erythropoietin using hypoxia-response enhancer | p. 139 |
| Direct control of cell metabolism by physicochemical conditions to increase productivity of glycosylated proteins | p. 143 |
| Large scale cGMP manufacturing of pDNA for gene therapy | p. 151 |
| A novel application of granulation technology to improve physical properties and biological performance of powdered serum-free culture media | p. 155 |
| High level production and characterization of recombinant human Thrombopoietin in CHO cells | p. 161 |
| Characterization of recombinant transglutaminase 1 and 3 expressed in baculovirus system | p. 167 |
| Construction of improved mammalian expression vectors | p. 173 |
| Immunoglobulin production stimulating activity of heat-denatured lysozyme | p. 179 |
| Enhancement of monoclonal antibody production of human hybridomas by retinoids | p. 185 |
| CB.Hep-1 hybridoma growth and antibody production using protein-free medium in a hollow fiber bioreactor | p. 189 |
| Production of Mab CB.HEP-1 for the purification of rHBsAg at high scale | p. 195 |
| Functional Cell Lines | |
| Establishment of novel cell line for bioartificial liver | p. 201 |
| Glycoengineering | |
| Improvement of sialylation in chinese hamster ovary (CHO) cell culture by feeding N-acetylmannosamine | p. 207 |
| Immunologicals, Monoclonal Antibodies, and Vaccines | |
| The role dendritic cells play in the cytokine responses in Peyer's patches | p. 213 |
| Characterization of cancer specific human monoclonal IgM antibodies produced by hybridomas derived from a patient with adult T-cell leukemia | p. 219 |
| Production of genetically engineered antigen specific human monoclonal IgE antibody | p. 225 |
| CD8[superscript +] T cells negatively regulate the antibody production from the human PBMC being immunized in vitro with antigen | p. 231 |
| Transplantation, Artificial Organs, and Organ Substitutes | |
| Effect of biomaterials and nutrient factors on chondrogenesis of human chondrocytes | p. 235 |
| Polarity and bile canaliculi-like channels in self-assembled rat hepatocyte spheroids | p. 241 |
| A tissue inducible small diameter vascular graft coated with PAU (copolymer of amino-acid and urethane) | p. 247 |
| Studies on the biocompatibility of artificial organs and tissue engineered products: Embryonic neuronal cell differentiation on the various kinds of biodegradable polymeres | p. 253 |
| Analysis of immunosuppressive factors expressed in serum of liver transplanted rats | p. 257 |
| Expression and amplification of glutamine synthetase gene for constructing ammonia-metabolizing cell lines in hybrid bioartificial liver support system | p. 263 |
| Reversion of transformed phenotype of polyetherurethane-induced tumor cells by Cx43 transfection | p. 269 |
| Transgenic Animals | |
| Expression of exogenous genes at the germinal ridge of germline chimeric chickens | p. 275 |
| Possible transfer of intra-testicularly injected foreign DNA to generations in chicken | p. 281 |
| Production of transgenic birds using pantropic retroviral vectors | p. 287 |
| Use of cultured primordial germ cells for production of transgenic fish | p. 293 |
| Safety and Regulation | |
| Analysis of antigenicity of collagenase-treated gelatin with antibodies secreted by immortalized human B cells | p. 299 |
| Cell Regulatory Factors and Signal Transduction | |
| A chromatin remodeling factor, BRM has important roles in cell differentiation | p. 303 |
| Human amniotic epithelial (HAE) cells produce erythropoietin | p. 309 |
| Antiproliferative and growth-stimulating activities of synthetic cytokinin analogs | p. 315 |
| Modulation of cell proliferation by the over-expression of the c-myc gene | p. 321 |
| Pleiotrophic functions of erythropoietin and tissue-specific regulation of its expression | p. 325 |
| Characteristics of orally tolerized CD4 T cells | p. 331 |
| Lysozyme-dependent proliferation switch: Hematopoietic and hybridoma cell growth control with the use of antibody/gp130 chimera | p. 337 |
| Human fibroblast growth factor binding protein HBp17 enhances the tumorigenic potential of immortalized squamous epithelial cells | p. 343 |
| The protective anti-oxidant effects of Kefir on SFME neural stem cells | p. 353 |
| The effect of high electromagnetic fields on the growth characteristics of human cell lines | p. 359 |
| Downregulation of high affinity IgE receptor Fc[varepsilon]RI expression in the human basophilic KU812 cells by a tea catechin | p. 365 |
| Putative protection mechanism of CTL from killing by their own perforin | p. 371 |
| Identification of differentially expressed genes in atopic dermatitis-prone NC/Nga mouse | p. 379 |
| Oxidative stress affects telomere maintenance in cancer cells | p. 385 |
| Functional Substances in Food and Natural Sources | |
| Suppression of hepatoma cell invasion in culture by resveratrol and its modes of action | p. 389 |
| Mechanisms of invasion inhibition of hepatoma cells by tea components and sera from tea-fed rats | p. 395 |
| A protein derived from Flammulina velutipes that enhances paracellular permeability of Caco-2 intestinal epithelial cells | p. 401 |
| Long term neurite outgrowth enhancing effect and neurite regeneration effect of an active substance from a brown alga, Sargassum macrocarpum on rat pheochromocytoma PC12D cells | p. 407 |
| Anti-allergic catechins of tea (Camellia Sinensis) | p. 415 |
| Bioavailabilities and inhibitory actions of trigonelline, chlorogenic acid and related compounds against hepatoma cell invasion in culture and their modes of actions | p. 421 |
| Changes in the relevant test parameters of 101 diabetes patients under the influence of the so-called "Nordenau-Phenomenon" | p. 427 |
| Effect of food component on differentiation and maturation of pre-adipocyte | p. 433 |
| Inhibitory effect of a chinese plant Saussurea inrolucrata on hyaluronidase and on [beta]-hexosaminidase release from rat basophilic leukemia (RBL-2H3) cells | p. 439 |
| Apoptosis and receptor-mediated signal regulation of cultured tumors by glycoprotein from Meretrix lusoria | p. 443 |
| Immue bioactive substances from fish larvae "Bull-Ary", Engraulis japonicus | p. 449 |
| Disease-preventing activities of plant products | p. 455 |
| Gamma-linolenic acid suppresses IgE production in vitro as well as in vivo | p. 461 |
| Regulation of serum leptin level by dietary conjugated linoleic acid in Sprague-Dawley rats | p. 467 |
| Apoptosis induction and cell cycle arrest in T-lymphoblastic leukemic cells (CEMSS) by damnacanthal | p. 473 |
| Animal Cells for in vitro Assay | |
| An in vitro approach to evaluation of the cross talk between intestinal epithelial cells and macrophages | p. 479 |
| Alkylphenolic compounds and their effect on the injury rate, survival and acetylcholinesterase activity of the rat reuronal cell line PC12 | p. 485 |
| Author Index | p. 491 |
| Subject Index | p. 497 |
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