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Advances in Chromatography : Volume 19 - J. Calvin Giddings

Advances in Chromatography

Volume 19

By: J. Calvin Giddings (Editor)

Hardcover Published: 1st February 1981
ISBN: 9780824712464
Number Of Pages: 312

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Contributorsp. iii
Contents of Other Volumesp. ix
Roles of High-Performance Liquid Chromatography in Nuclear Medicinep. 1
Introductionp. 2
Characteristics of Radiopharmaceuticalsp. 3
Instrumentationp. 5
Preparation and Purificationp. 6
Analysis of [superscript 99m]Tc-Labeled Pharmaceuticalsp. 28
Conclusionp. 31
Acknowledgmentsp. 32
Referencesp. 32
Calibration of Separation Systems in Gel Permeation Chromatography for Polymer Characterizationp. 37
Introductionp. 38
Calibration Using Standards with Narrow Distributionsp. 39
Calibration Using Standards with Broad Distributionp. 46
Universal Calibrationp. 52
Special Procedures of Calibrationp. 71
Influence of Operational Variables on Results of Calibrationp. 72
Conclusionsp. 78
Acknowledgmentsp. 79
Applications of Universal Calibration Principles in Synthetic Polymer Characterizationp. 80
Referencesp. 80
Isomer-Specific Assay of 2,4-D Herbicide Products By HPLC: Regulatory Methodologyp. 91
Introductionp. 91
Ion-Exchange Chromatography Versus Reverse-Phase Chromatographyp. 93
Method Criteriap. 94
Impurities in 2,4-Dp. 94
Separation of Impuritiesp. 94
Alternative Columnsp. 100
Column Stabilityp. 107
Precision and Linearityp. 107
Mobile Phase Modifierp. 108
Conclusionsp. 108
Referencesp. 108
Hydrophobic Interaction Chromatographyp. 111
Introductionp. 112
Definition of Hydrophobic Interaction and the Structure of Waterp. 112
Changes in the Water Structure upon Solubilization of a Nonpolar Compound in Waterp. 113
Chemical Nature of the Hydrophobic Interactionp. 113
Charged Amphiphilic Adsorbentsp. 115
Neutral Amphiphilic Adsorbentsp. 116
Multipoint Interactionp. 117
Establishment of Appropriate Experimental Conditions for a Runp. 118
Applicationsp. 119
Acknowledgmentsp. 121
Referencesp. 121
Liquid Chromatography with Programmed Composition of the Mobile Phasep. 125
Introductionp. 126
Retention Characteristics in Isocratic-Elution Chromatography and in Chromatography with Programmed Composition of the Mobile Phasep. 128
Practical Aspects of Chromatography with Programmed Composition of the Mobile Phasep. 235
Conclusionsp. 248
Summaryp. 248
List of Symbols Usedp. 249
Referencesp. 253
Chromatographic Separation of Aldosterone and Its Metabolitesp. 261
Introductionp. 262
Metabolism and Mechanism of Action of Aldosteronep. 263
Separation and Column Chromatography of Reduced and Polar Aldosterone Metabolitesp. 264
High-Pressure Liquid Chromatography of Reduced and Polar Aldosterone Metabolitesp. 268
Improved Separations of Aldosterone Metabolites Using High-Pressure Liquid Chromatographyp. 273
High-Pressure Liquid Chromatography of Aldosterone Metabolites Synthesized in Vitrop. 275
Aldosterone Metabolites in Male Rat Urinep. 281
Conclusionsp. 282
Acknowledgmentsp. 283
Referencesp. 283
Author Indexp. 287
Subject Indexp. 307
Table of Contents provided by Ingram. All Rights Reserved.

ISBN: 9780824712464
ISBN-10: 0824712463
Series: Advances in Chromatography : Book 19
Audience: Tertiary; University or College
Format: Hardcover
Language: English
Number Of Pages: 312
Published: 1st February 1981
Publisher: TAYLOR & FRANCIS
Country of Publication: US
Dimensions (cm): 23.37 x 15.14  x 2.16
Weight (kg): 0.61
Edition Number: 1

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