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A Practical Guide to the Study of Calcium in Living Cells : Volume 40 - Leslie Wilson

A Practical Guide to the Study of Calcium in Living Cells

Volume 40

By: Leslie Wilson (Editor), Paul T. Matsudaira (Editor), Richard Nuccitelli (Editor)

Paperback

Published: 18th May 1994
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A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don'ts and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Key Features
* Describes techniques for detection of [Ca2+]i: Ca2+ - sensitive microelectrodes
* fluorescent dyes
* luminescent proteins
* Includes techniques for perturbing intracellular Ca2+
* Covers detailed methodology plus problems and pitfalls of each technique
* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
* Color plates illustrate techniques such as
* Confocal ratio-imaging
* Use of aequorin

"This multiauthored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca2+]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced...The book provides a pragmatic approach for the estimation of cytosolic [Ca2+]i and also provides a means for studying the spatial and temporal resolution of [Ca2+]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research." --RAMESH BHALLA, University of Iowa, Iowa City "The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research." --MOLECULAR & CELLULAR NEUROSCIENCES

Ca2+ Buffers and [Ca2+]I Perturbation Techniques
A Practical Guide to the Preparation of Ca2+ Buffers
Photorelease Techniques for Raising or Lowering Intracellular Ca2+
Pharmacological Tools for Perturbing Intracellular Calcium Storage
Microelectrode Techniques for Measuring [Ca2+]I and Ca2+ Fluxes
How to Make and to Use Calcium-Specific Mini- and Microelectrodes
The Vibrating Ca2+
Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux
IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels
Fluorescence Techniques for Imaging [Ca2+]I
Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators
Rapid Simultaneous Estimation of Intracellular Calcium and pH
Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques
Confocal Imaging of Ca2+ in Cells
Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes
Use of Aequorin for [Ca2+]I Imaging
Inexpensive Techniques for Measuring [Ca2+]I Changes Using a Photomultiplier Tube
Imaging [Ca2+]I with Aequorin Using a Photon Imaging Detector
Targeting Recombinant Aequorin to Specific Intracellular Organelles
Index
Table of Contents provided by Publisher. All Rights Reserved.

ISBN: 9780125228107
ISBN-10: 0125228104
Series: Methods in Cell Biology
Audience: Professional
Format: Paperback
Language: English
Number Of Pages: 368
Published: 18th May 1994
Publisher: Elsevier Science Publishing Co Inc
Country of Publication: US
Dimensions (cm): 24.0 x 19.1  x 2.0
Weight (kg): 0.66