A Practical Guide to Protein and Peptide Purification for Microsequencing - Paul T. Matsudaira

A Practical Guide to Protein and Peptide Purification for Microsequencing

By: Paul T. Matsudaira (Editor)

Paperback | 20 May 1993 | Edition Number 2

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Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
  • Mass spectrophotometric characterization of proteins and peptides
  • N-terminal sequencing of proteins with blocked N termini
  • Internal amino acid sequence analysis after protease digestion in-gel and on-blot
  • Improved microscale peptide purification methods
  • Computer analysis of protein sequences
  • New protocols tested and refined through everyday use in authors' laboratories
  • Updated reference chapter covering all aspects of protein microsequencing
Industry Reviews

Reviews of the First Edition: "Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell

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