This book provides a state-of-the-art approach to the molecular basis of hematologic diseases and its translation into improved diagnostics and novel therapeutic strategies. Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms. Experts in the field describe the molecular methods applied for modern diagnostics and therapies, such as hematopoietic stem cell transplantation, donor recipient matching, banking of biological material, analyses of post-transplant chimerism, and minimal residual disease monitoring. The volume concludes with an extensive section comprising thorough step-by-step protocols of molecular techniques in hematology, all of them validated in the authors' own laboratories.
From the reviews: "This book describes current diagnostic tools and clinical procedures used for hematological malignancies. ... The goal was to meet the needs of both clinical hematologists who treat patients with leukemia, lymphoma, and/or multiple myeloma and those who study these diseases in the laboratory. ... an excellent review of molecular biology for specific hematological malignancies and disorders. It also covers important molecular testing techniques that are commonly used in the diagnosis, management, and surveillance of these malignancies. ... a valuable addition to any hematologist's library." (John Patrick Galvin, Doody's Review Service, October, 2012)
Part I MOLECULAR BIOLOGY OF SELECTED HEMATO-ONCOLOGIC DISEASES 1. Molecular biology of acute lymphoblastic leukemia Malgorzata Dawidowska, Monika D. Kraszewska, Katarzyna Derwich, and Tomasz Szczepanski 2. Genetic mechanisms and molecular markers of neoplastic transformation in acute myeloid leukemia Agata A. Filip, Marta Libura, Sebastian Giebel, and Olga Haus 3. Molecular pathogenesis of aggressive B-cell lymphomas Przemyslaw Juszczynski and Krzysztof Warzocha 4. Molecular biology and classification of multiple myeloma Anna Dmoszynska and Norbert Grzasko 5. Chronic lymphocytic leukemia Anton W. Langerak and Yorick Sandberg 6. The molecular biology of chronic myeloid leukemia Tomasz Sacha, Kajetana Foryciarz, and Aleksander B. Skotnicki 7. Molecular biology of myelodysplastic syndromes Francesc Sole 8. Myeloproliferative neoplasms Andrzej Hellmann, Maria Bieniaszewska, Witold Prejzner, and Aleksandra Leszczynska Part II DIAGNOSTICS AND MONITORING OF THERAPY OF HEMATO-ONCOLOGIC DISEASES 9. Flow cytometric immunophenotyping as diagnostic tool of hematopoietic malignancies Lukasz Sedek, Juan Flores-Montero, Joanna Bulsa, Susana Barrena, Julia Almeida, Alberto Orfao, and Tomasz Szczepanski 10. Cytogenetics in hematology Olga Haus, Anna Poluha, and Katarzyna Skonieczka 11. Monitoring of minimal residual disease in acute lymphoblastic leukemia Tomasz Szczepanski, Malgorzata Dawidowska, and Katarzyna Derwich 12. Gene expression profiling in hematologic malignancies Przemyslaw Juszczynski, Bjoern Chapuy, Malgorzata Szczepaniak, and Krzysztof Warzocha Part III MOLECULAR PROCEDURES INVOLVED IN HEMATOPOIETIC STEM CELL TRANSPLANTATION 13. Allogeneic transplantation of hematopoietic stem cells Jacek Wachowiak 14. Genetic basis of donor-recipient matching in allogeneic transplantation of hematopoietic stem cells Jacek Nowak and Jacek Wachowiak 15. Chimerism following allogeneic transplantation of hematopoietic stem cells Malgorzata Dawidowska, Katarzyna Guz, Ewa Brojer, Jacek Wachowiak, and Michal Witt 16. Peritransplantation monitoring of minimal residual disease in acute lymphoblastic leukemia Malgorzata Dawidowska, Katarzyna Derwich, and Tomasz Szczepanski 17. Biobanks of cellular material Wieslaw Wiktor-Jedrzejczak and Elzbieta Urbanowska Part IV MOLECULAR DIAGNOSTIC PROTOCOLS 18. Biobanking of cellular material Anna Poluha and Elzbieta Urbanowska 19. Isolation of mononuclear cells from human blood and bone marrow by density gradient centrifugation Malgorzata Dawidowska 20. Molecular techniques commonly used in hematooncolology Monika D. Kraszewska and Ewa Zietkiewicz 21. Genetic methods of HLA typing Jacek Nowak, Renata Mika-Witkowska, and Elzbieta Graczyk-Pol 22. Post-transplant chimerism analysis through STR-PCR and RQ-PCR Malgorzata Dawidowska, Katarzyna Guz, Agnieszka Orzinska, Justyna Smolarczyk-Wodzynska, Monika D. Kraszewska, and Michal Witt 23. Analysis of minimal residual disease with the use of rearrangements of Ig/TCR genes through RQ-PCR M. Dawidowska, Vincent H. J. van der Velden, Michal Witt, and Tomasz Szczepanski 24. Molecular diagnostics of acute myeloblastic leukemia Marta Libura, Agata A. Filip, and Olga Haus 25. Assessment of the presence and the level of BCR-ABL fusion gene expression and mutational status in ABL kinase domain. Sylwia Czekalska, Magdalena Zawada, and Izabela Florek 26. Studies of rearrangements and somatic hypermutation of IGHV genes in chronic lymphocytic leukemiaAnton W. Langerak, Richard Rosenquist, Paolo Ghia, Chrysoula Belessi, Kostas Stamatopoulos, and Frederic Davi for the ERIC-IgCLL group 27. Molecular biology methods in the diagnosis of multiple myeloma Anna Dmoszynska and Sylwia Chocholska 28. Molecular methods in myeloproliferative neoplasms Aleksandra Leszczynska, Witold Prejzner, Maria Bieniaszewska, and Andrzej Hellmann 29. Informed consent for participation in research project Malgorzata Dawidowska and Michal Witt
Series: Principles and Practice
Number Of Pages: 486
Published: 1st July 2012
Dimensions (cm): 23.5 x 15.5
Weight (kg): 0.89