Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology. Written in the Methods in Molecular Biology(t) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.
1. Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology Ana Maria Fraga, Erica Sara Souza de Araujo, Raquel Stabellini, Naja Vergani, and Lygia V. Pereira 2. Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview Udaykumar Kolkundkar, Mohan Vemuri, Geetha.M.Swamilingiah, Shruthi Pal, and Jasmeet Kaur 3. Human Embryonic Stem Cells Derived in Xeno-Free Conditions Amparo Galan Albinana and Carlos Simon Valles 4. Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark Linda Harkness and Moustapha Kassem 5. Principles for Derivation of Human Embryonic Stem Cells Mikael C.O. Englund, Catharina Ellerstrom, Katarina Andersson, Karin Noaksson, and Johan Hyllner 6. Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells Guoliang Meng and Derrick E. Rancourt 7. Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos Ileana Mateizel, Mieke Geens, Hilde Van de Velde, and Karen Sermon 8. Analysis of LINE-1 Expression in Human Pluripotent Cells Martin Munoz-Lopez, Marta Garcia-Canadas, Angela Macia, Santiago Morell, and Jose L. Garcia-Perez 9. Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan Steven Shoei-Lung Li 10. Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos Ronak Shetty and Maneesha S. Inamdar 11. Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos Juan-Carlos Biancotti and Neta Lavon 12. Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, and Dalit Ben-Yosef 13. Single-Cell Enzymatic Dissociation of hESC Lines OxF1--OxF4 and Culture in Feeder-Free Conditions Frances A. Brook 14. Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension Hossein Baharvand, Mehran Rezaei Larijani, and Maryam Yousefi 15. Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells Roman Krawetz and Derrick E. Rancourt 16. Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs) Khun-Hong Lie, Henry Chung, and Kuldip S. Sidhu 17. Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol Dezhong Yin, Tahereh Tavakoli, Wei-Qiang Gao, and Wu Ma 18. Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells Aaron M. Elliott, Kristi A. Hohenstein Elliott, and Anja Kammesheidt 19. Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells Marc Jung and James Adjaye 20. Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq) Christina Grimm and James Adjaye 21. Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis Linda Harkness, Tatyana A. Prokhorova, Moustapha Kassem, and Blagoy Blagoev
Series: Methods in Molecular Biology (Hardcover)
Published: 20th April 2012
Dimensions (cm): 25.4 x 18.542
Weight (kg): 0.771