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Correlative Light and Electron MIcroscopy

Correlative Light and Electron MIcroscopy

By: Thomas Mueller-Reichert (Editor), Paul Verkade (Editor)

Hardcover

Published: 24th August 2012
Ships: 7 to 10 business days
RRP $323.99
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The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

  • Covers many areas of CLEM by the best international scientists in the field
  • Includes a brief history and overview on CLEM methods

    • Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
    Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy
    Correlative Fluorescence and Transmission Electron Microscopy in Tissues
    Correlative Light and Electron Microscopy in Parasite Research
    Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy 3D HDO-CLEM: Cellular ComPartment Analysis by Correlative Light-Electron Microscopy on Cryosection
    Correlative Light and Electron Microscopy of GFP
    Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
    Correlated Light Microscopy and Electron Microscopy (CLEM): Search. and Find!
    Capturing Endocytic Segregation Events with HPF-CLEM
    Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
    Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
    Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
    Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
    Visualizing Proteins in Electron Micrographs at Nanometer Resolution
    Atmospheric Scanning Electron Microscope for Correlative Microscopy
    Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
    Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms
    Table of Contents provided by Publisher. All Rights Reserved.

    ISBN: 9780124160262
    ISBN-10: 0124160263
    Audience: Professional
    Format: Hardcover
    Language: English
    Number Of Pages: 460
    Published: 24th August 2012
    Dimensions (cm): 23.5 x 19.1  x 3.0
    Weight (kg): 1.247