Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Key Features
Highlights of the Second Edition:
* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing
From the Reviews of the First Edition "Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --CELL
| Introduction | |
| Strategies for Obtaining Partial Amino Acid | |
| Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein | |
| Enzymatic Digestion of Proteins and HPLC Peptide Isolation | |
| Purification of Proteins and Peptides By SDS-PAGE | |
| Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose | |
| Mass Spectrometric Strategies for the Structural Characterization of Proteins | |
| Appendix | |
| References | |
| General Reference | |
| Books on Protein Microsequencing Methodologies | |
| Table of Contents provided by Publisher. All Rights Reserved. |
ISBN: 9780124802827
ISBN-10: 0124802826
Audience:
Professional
Format:
Paperback
Language:
English
Number Of Pages: 184
Published: 3rd June 1993
Dimensions (cm): 22.9 x 15.2
x 1.1
Weight (kg): 0.291